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Plasmid#172755PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-35
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-19
Plasmid#172748PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-19
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-3
Plasmid#172740PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-3
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-12
Plasmid#172744PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-12
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-14
Plasmid#172745PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-14
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
APEX2-csGBP
Plasmid#108874PurposeExpression of APEX2 fused to a conditionally stable nanobody against GFPDepositorInsertAPEX2-csGBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
APEX2-csChBP
Plasmid#108876PurposeExpression of APEX2 fused to a conditionally stable nanobody against mCherryDepositorInsertAPEX2-csChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
APEX2-ChBP
Plasmid#108878PurposeExpression of APEX2 fused to a nanobody against mCherryDepositorInsertAPEX2-ChBP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMito-GBPen
Plasmid#128269PurposeExpression of mitochondrially targeted GBPen (GFP Nanobody)DepositorInsertMito-GBPen
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3E-ChBP
Plasmid#108892PurposeMultisite gateway vector for 3' tagging with a nanobody against mCherryDepositorInsertChBP
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceJune 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
MARS-nGFP
Plasmid#205234PurposeExpresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cellsDepositorInsertPLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
UseTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAK464
Plasmid#140702PurposeNBαEGFPDepositorInsertNBαEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAK463
Plasmid#140701PurposeNBαMs-IgKDepositorInsertNBαMs-IgK
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-26b-NbVHH05-HA-His
Plasmid#171567PurposeBacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)DepositorInsertNbVHH05-HA-His (UBE2J1 Human)
UseTagsHA-tag/His-tag and PelB signal peptideExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-Nb127D01-mCherry
Plasmid#171573PurposeExpresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cellsDepositorInsertNb127D01-mCherry (CXCR2 Human)
UseTagsmCherryExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-NbVHH05-mCherry
Plasmid#171572PurposeExpresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cellsDepositorInsertNbVHH05-mCherry (UBE2J1 Human)
UseTagsmCherryExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
(383) pcDNA3.1-HA-nBC2-OGA(544-706)
Plasmid#168100PurposeHA- tagged BC2 nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGADepositorInsertHA-nBC2-OGA(544-706)
UseTagsHA-tagExpressionMammalianMutationOGA truncated to contain residues 544-706PromoterT7/CMVAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
(344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)
Plasmid#168097PurposeHA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.DepositorInsertHA-nGFP-(EAAAK)4-OGA(544-706)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-CD8-Nb127D01-GFP
Plasmid#171577PurposeExpresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembraneDepositorInsertCD8-Nb127D01-GFP (CXCR2 Human)
UseTagsCD8 and GFPExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAW-Nb127D01-GFP
Plasmid#171571PurposeExpresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cellsDepositorInsertNb127D01-GFP (CXCR2 Human)
UseTagsGFPExpressionInsectMutationPromoterfly actin5C promoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only