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Plasmid#208945PurposeA control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…PromoterCMV and T7Available sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET StrepII-StrepII TEV LIC cloning vector (2RRT)
Plasmid#55215DepositorTypeEmpty backboneUseTagsStrepII-StrepIIExpressionBacterialMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Gamma crystallin TEV LIC cloning vector (1X)
Plasmid#29661DepositorTypeEmpty backboneUseTagsHis6-Gamma crystallin-TEVExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 StrepII TEV LIC cloning vector (2HR-T)
Plasmid#29718DepositorTypeEmpty backboneUseTagsHis6-StrepII-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)
Plasmid#30184DepositorTypeEmpty backboneUseTagsBiotin-H6-TEV recognition and FlAsHExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mClover LIC cloning vector (MBP-mClover)
Plasmid#86930PurposepET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mCloverDepositorTypeEmpty backboneUseTagsBiotin, His6, MBP, Tev Recognition Sequence, and …ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mOrange LIC cloning vector (MBP-mOrange)
Plasmid#29748DepositorTypeEmpty backboneUseTagsBiotin - His6 - MBP -TEV and mOrangeExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mCerulean LIC cloning vector (MBP-mCerulean)
Plasmid#29751DepositorTypeEmpty backboneUseTagsBiotin - His6 - MBP -TEV and mCeruleanExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET MBP-FlAsH LIC cloning vector (MBP-FlAsH)
Plasmid#30185DepositorTypeEmpty backboneUseTagsBiotin-H6-MBP-TEV recognition and FlAsHExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Gamma crystallin TEV LIC cloning vector (2X-T)
Plasmid#29714DepositorTypeEmpty backboneUseTagsHis6-Gamma crystallin-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET MBP tdTomato LIC cloning vector (MBP-tdTomato)
Plasmid#86927PurposepET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-tdTomatoDepositorTypeEmpty backboneUseTagsBiotin, His6, MBP, Tev Recognition Sequence, and …ExpressionBacterialMutationPromoterT7Available sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mCitrine LIC cloning vector (MBP-mCitrine)
Plasmid#29749DepositorTypeEmpty backboneUseTagsBiotin - His6 - MBP -TEV and mCitrineExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R
Plasmid#166814PurposeMammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activityDepositorInsertNLRP1 (NLRP1 Human)
UseTagsFLAGExpressionMammalianMutationE1322RPromoterCMVAvailable sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R K1277E
Plasmid#166811PurposeMammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association, K1277E abolishes UPA-UPA contactsDepositorInsertNLRP1 (NLRP1 Human)
UseTagsFLAGExpressionMammalianMutationLL1193EE, P1214R, K1277EPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG H1276G
Plasmid#166812PurposeMammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activityDepositorInsertNLRP1 (NLRP1 Human)
UseTagsFLAGExpressionMammalianMutationH1276GPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG K1277E
Plasmid#166813PurposeMammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activityDepositorInsertNLRP1 (NLRP1 Human)
UseTagsFLAGExpressionMammalianMutationK1277EPromoterCMVAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS014E: Entry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)
Plasmid#163072PurposeEntry vector for: Grx1-roGFP2 glutathione redox sensor (cytosolic)DepositorInsertGrx1-roGFP2
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5C)
Plasmid#30123DepositorTypeEmpty backboneUseTagsHis6-MBP-N10-TEVExpressionInsectMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)
Plasmid#48294DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)
Plasmid#55209DepositorTypeEmpty backboneUseTagsHis10 and MPB, Asn10ExpressionBacterialMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only