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Plasmid#169421PurposeDonor DNA for TKIT knockin of SEP to GluA2 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDonor-tBFP-NLS-Neo
Plasmid#80766PurposeCustomizable donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.DepositorTypeEmpty backboneUseCRISPRTagsTagBFP2-3×NLSExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
mini-donor - NKX6.1-nEGFP
Plasmid#206047Purposethis mini-donor knockin vector can be used to knock nuclear EGFP into NKX6.1 locusDepositorInsertnuclear EGFP
UseTagsExpressionMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE HA-epitope donor ORF0
Plasmid#200371PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE HA-epitope donor ORF2
Plasmid#200373PurposeTagging endogenously expressed proteins with HA epitope, C-terminalDepositorInsertHA epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE smFP-V5 donor ORF0
Plasmid#200380PurposeTagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminalDepositorInsertspaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE smFP-V5 donor ORF1
Plasmid#200381PurposeTagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminalDepositorInsertspaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
mini-donor - SOX17-EGFP
Plasmid#206048Purposethis mini-donor knockin vector can be used to knock EGFP into SOX17 locusDepositorInsertnuclear EGFP
UseKnockin donor vectorTagsExpressionMutationPromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS Donor mClover-LMNA
Plasmid#98970PurposeDonor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination ReporterDepositorInsertLMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONOR223 RpH-LAMP1-3xFLAG
Plasmid#163019Purposegateway donor vector encoding ratiometric sensor of lysosomal lumenal pHDepositorInsertRpH-LAMP1-3xFLAG (Lamp1 Mouse)
UseGateway systemTags3XFLAGExpressionMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluA1 TKIT donor
Plasmid#169418PurposeDonor DNA for TKIT knockin of SEP to GluA1 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHFS:SEP-rGluA1 TKIT donor
Plasmid#169438PurposeDonor DNA for TKIT knockin of SEP to GluA1 in ratDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHFS:SEP-rGluA2 TKIT donor
Plasmid#169440PurposeDonor DNA for TKIT knockin of SEP to GluA2 in ratDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-tagBFP-PSM-EGFP
Plasmid#100603PurposeDonor scaffold with EGFP positive selection module, and tagBFP negative selection moduleDepositorTypeEmpty backboneUseSynthetic BiologyTagsEGFP-T2A-Puro and tagBFPExpressionMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-AcHELIX-KanR (CJT96)
Plasmid#181794PurposepDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONOR-tagBFP-PSM-dTOMATO
Plasmid#100604PurposeDonor scafold with dTOMATO positive selection module, and tagBFP negative selection moduleDepositorTypeEmpty backboneUseSynthetic BiologyTagsdTOMATO-T2A-Puro and tagBFPExpressionMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL3936 (pDonor(AAVS1)_Pse)
Plasmid#200903PurposeEncodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.DepositorInsertMini Tn7016 (AAVS1 NGS)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluN1 TKIT donor
Plasmid#169427PurposeDonor DNA for TKIT knockin of SEP to NR1 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHiUGE V5-epitope donor ORF1
Plasmid#200378PurposeTagging endogenously expressed proteins with V5 epitope, C-terminalDepositorInsertV5-epitope
UseTagsExpressionMammalianMutationNAPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:SEP-GluA3 TKIT donor
Plasmid#169425PurposeDonor DNA for TKIT knockin of SEP to GluA3 in mouseDepositorInsertSuper ecliptic pHluorin (SEP)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only