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Showing: 381 - 400 of 4623 results

  5. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  6. pET24a-ncOGT-TPR

    Plasmid
    #190822
    Purpose
    For expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    C467X. Also codon optimized for expression in E.c…
    Promoter
    T7
    Availability
    Industry, Academic Institutions, and Nonprofits
    190822-plasmid-map-sequence-id-379182

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  8. pL

    Plasmid
    #196286
    Purpose
    35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV L antigenome encoding codon-optimized L (RdRp)
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Availability
    Academic Institutions and Nonprofits only
    196286-plasmid-map-sequence-id-390014

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  10. pCG_SARS-CoV2 NSP7/bat-CoV RaTG13 C-V5-tag

    Plasmid
    #179974
    Purpose
    Mammalian expression vector for SARS-CoV-2 Nsp7 or bat RaTG13 Nsp7, V5-tagged (SARS-CoV-2 Nsp7 and RaTG13 Nsp7 have the same amino acid sequence and codon optimized sequence).
    Depositor
    Konstantin Sparrer
    Article
    Hayn et al Cell Rep. 2021 May 18;35(7):109126.
    Insert
    SARS-CoV-2 Nsp7 (ORF1ab Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    human codon-optimized
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    179974-plasmid-map-sequence-id-353863

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  12. pORFE0001

    Plasmid
    #112070
    Purpose
    AtCas9 module CDS1
    Depositor
    Christian Chevalier
    Insert
    AtCAS9
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized Cas9 S. pyogenes
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    112070-plasmid-map-sequence-id-217624

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  13. FRP2008

    Plasmid
    #159312
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized mKOkappa under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    V2del
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    159312-plasmid-map-sequence-id-311212

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  14. FRP2010

    Plasmid
    #159313
    Purpose
    Yeast tagging vector to create gene fusion with yeast codon optimized tSapphire under KanMX selection
    Depositor
    Joerg Stelling
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    V2del, L231H
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    159313-plasmid-map-sequence-id-311213

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Showing: 381 - 400 of 4623 results