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Plasmid#188499PurposeExpresses FLAG tagged SpdCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/H840APromoterEF1aAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas12j2 KRAB
Plasmid#188508PurposeExpresses FLAG tagged dCas12j2 KRABDepositorInsertdCas12j2
UseCRISPR and LentiviralTagsExpressionMammalianMutationD394APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
SadCas9 KRAB
Plasmid#188504PurposeExpresses FLAG tagged SadCas9 KRABDepositorInsertdCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A/N580APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AsdCas12a KRAB
Plasmid#188502PurposeExpresses FLAG tagged AsdCas12a KRABDepositorInsertdCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationD908APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LbdCas12a VPR
Plasmid#188513PurposeExpresses FLAG tagged LbdCas12a VPRDepositorInsertdCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationD832APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
miniCMV eGFP hPGK BSD
Plasmid#188519PurposeBackbone for CRISPR activation (CRISPRa) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LbdCas12a KRAB
Plasmid#188503PurposeExpresses FLAG tagged LbdCas12a KRABDepositorInsertdCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationD832APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro Non-targeting sgRNA 3
Plasmid#109003PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #3
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a
Plasmid#188493PurposeExpresses FLAG tagged LbCas12aDepositorInsertCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9
Plasmid#188489PurposeExpresses FLAG tagged SpCas9DepositorInsertCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro Non-targeting sgRNA 1
Plasmid#109002PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #1
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
EFS eGFP hPGK BSD
Plasmid#188520PurposeBackbone for CRISPR interference (CRISPRi) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR-V2_mMSH2
Plasmid#186156PurposesgRNA targeting murine Msh2 geneDepositorInsertMsh2_gRNA (Msh2 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas12j3 VPR
Plasmid#188517PurposeExpresses FLAG tagged dCas12j3 VPRDepositorInsertdCas12j3
UseCRISPR and LentiviralTagsExpressionMammalianMutationD413APromoterEF1aAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas12j3 KRAB
Plasmid#188507PurposeExpresses FLAG tagged dCas12j3 KRABDepositorInsertdCas12j3
UseCRISPR and LentiviralTagsExpressionMammalianMutationD413APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
dCas12j2 VPR
Plasmid#188518PurposeExpresses FLAG tagged dCas12j2 VPRDepositorInsertdCas12j2
UseCRISPR and LentiviralTagsExpressionMammalianMutationD394APromoterEF1aAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
V1-MESA-45F-M-dCas9
Plasmid#84504PurposeMESA target chain with V1-MESA ectodomain, 45 extracellular linkers, a flag tag, M cleavage sequence, and dCas9-VP64DepositorInsertV2-MESA-35F-M-dCas9
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti SYN-SVI-dCas9-VPR
Plasmid#164575PurposeExpresses an intron-containing dCas9-VPR fusion driven by human SYN promoterDepositorInsertFLAG-dCas9-VPR containing an SV40 intron
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW213-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170811PurposeLentiviral vector to co-express an sasgRNA with NLS-mNeonGreenDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationNAPromoterAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXL_dSSN2_EF1a-tNGFR-2A-Puro
Plasmid#201112PurposeDual barcoding cassettes for SSN-seq; Barcode Set 2DepositorInsertsSSN.guide
SSN.F30
tNGFR
UseLentiviralTagsExpressionMutationPromoterEF1a, Human U6, and Mouse U6Available sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only