Addgene

We narrowed to 916 results for: THY-2

Showing: 381 - 400 of 916 results

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dCas9-10xSunTag-P2A-tagBFP

Plasmid

#174141

Purpose

Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

Depositor

Albert Jeltsch

Article

Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

Insert

dCas9, 10xSunTag, tagBFP

Use

Tags

HA and tagBFP

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 Srap1

Plasmid

#89691

Purpose

Expresses murine Srap1 in bacteria

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Murine SRAP domain ortholog Srap1 (Hmces Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pET30a-SRAP

Plasmid

#84524

Purpose

Expresses murine SRAP domain (250 aa) in bacteria

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

SRAP domain (Hmces Mouse)

Use

Tags

6xHis and S-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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AV4-EF1-GFP

Plasmid

#124971

Purpose

Control for AV4-EF1-NMNAT2

Depositor

Kazuhiro Oka

Article

Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

EF1-alpha

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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Banshee-ShCit-A

Plasmid

#85216

Purpose

Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

Depositor

Alex Minella

Article

Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

Insert

shCit-A

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Banshee-ShCit-B

Plasmid

#85217

Purpose

Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

Depositor

Alex Minella

Article

Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

Insert

shCit-B

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET30a-SRAP-delCys2

Plasmid

#84568

Purpose

Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Srap1 (Hmces Mouse)

Use

Tags

6xHis and S-Tag

Expression

Bacterial

Mutation

deletion of Cys2

Promoter

T7

Available since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pJS699

Plasmid

#168779

Purpose

Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Insert

Spike RBD (S betacoronavirus)

Use

Tags

Cmyc

Expression

Mutation

N343Q

Promoter

GAL1

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Pam-FL

Plasmid

#42570

Depositor

Vijaya Ramesh

Article

Santos et al J Neurosci Res. 2006 Feb 1;83(2):222-32.

Insert

human MYCBP2 (MYCBP2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Pam-FL clone lacks alternatively spliced exons 52…

Promoter

CMV

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3/HA-FLAG-RBP2

Plasmid

#14800

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3/HA-RBP2

Plasmid

#14799

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pYES260-sc.eEF2

Plasmid

#85119

Purpose

yeast expression of EEF2

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFT1/EFT2 (EFT2, EFT1 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBS cSugarCrisp (CT#708)

Plasmid

#13956

Depositor

Cliff Tabin

Article

Smith et al Mech Dev. 2001 Apr . 102(1-2):223-6.

Insert

SugarCrisp (PI15 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pBS hSugarCrisp (CT#592)

Plasmid

#13966

Depositor

Cliff Tabin

Article

Smith et al Mech Dev. 2001 Apr . 102(1-2):223-6.

Insert

SugarCrisp (PI15 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pSt1374-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS

Plasmid

#112210

Purpose

Targeted DNA methylation

Depositor

Xingxu Huang

Article

Lu et al Cell Death Dis. 2020 Feb 3;11(2):85. doi

Insert

DNA methyltransferase 3 alpha (DNMT3A, DNMT3L Human)

Use

CRISPR

Tags

Expression

Mutation

D10A,H840A,N863A

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pGL410_Ins2500

Plasmid

#49058

Purpose

Expresses luciferase under control of mouse insulin 2 gene promoter

Depositor

Kevin Ferreri

Article

Kuroda et al PLoS One. 2009 Sep 9;4(9):e6953. doi: 10

Insert

mouse Ins2 promoter (Ins2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ins2 promoter

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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GST-BHMT-I/G

Plasmid

#104442

Purpose

Autophagy cargo assay

Depositor

Carol Mercer

Article

Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

Insert

BHMT (BHMT Human)

Use

Tags

GFP-myc GFP is from Aequorea victoria and GST

Expression

Mammalian

Mutation

N43A in GST (in a potential KFERQ-like sequence).…

Promoter

CMV

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pFlc245

Plasmid

#101021

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

GluR 1 (Gria1 Rat)

Use

Tags

Expression

Mammalian

Mutation

fluorescent protein inserted into subunit

Promoter

CMV

Available since

Sept. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-puro/HA-FLAG-RBP2

Plasmid

#14802

Depositor

William Kaelin

Article

Klose et al Cell. 2007 Mar 9. 128(5):889-900.

Insert

Retinoblastoma Binding Protein 2 (KDM5A Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
Human HMG-CoA lyase (HL)

Plasmid

#52433

Purpose

bacterial expression of human HMG-CoA lyase (HL)

Depositor

Henry Miziorko

Article

Montgomery et al Arch Biochem Biophys. 2011 Jul;511(1-2):

Insert

HMG CoA lyase (HMGCL Human)

Use

Tags

His and S

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 8, 2014

Availability

Academic Institutions and Nonprofits only