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Plasmid#198445PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W69FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro
Plasmid#198434PurposeActivity/inhibition assays, 19F-NMRDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W50F
Plasmid#198444PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationR95*A, W50FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEG412
Plasmid#40087DepositorTypeEmpty backboneUseTagsDendra2 PAFP, S-peptide, and TEV protease cleavag…ExpressionWormMutationPromoterpie-1Available sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIC306
Plasmid#52257PurposeRetroviral expression of EGFP-TEV-S-tag SKA3 for protein localization and affinity purification (LAP)DepositorInsertSka3 (SKA3 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIC292
Plasmid#51405PurposeRetroviral expression of EGFP-TEV-S-tag SKA2 for protein localization and affinity purification (LAP)DepositorInsertSka2 IMAGE 4685972 (SKA2 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIC291
Plasmid#51403PurposeRetroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)DepositorInsertSka1 IMAGE 3909951 (SKA1 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
SUV420H2
Plasmid#32864PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSUV420H2 (KMT5C Human)
UseTagsHis tag (with TEV protease)ExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEG545
Plasmid#40116DepositorTypeEmpty backboneUseGateway donor vectorTagsDendra2 PAFP (sequence optimized for worm express…ExpressionMutationPromoterAvailable sinceSept. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
5ZiPro
Plasmid#198432PurposeConformation sensitive site specific Cys-labelingDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationS84*C, R95*A, C80S, C143S, S160C, C178S,PromoterT7 promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
gZiPro W61*F
Plasmid#198442PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertglycine linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW61*F, R95*A,PromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro W5F
Plasmid#198436PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW5FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro W83F
Plasmid#198439PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW83FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro W69F
Plasmid#198438PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW69FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro W89F
Plasmid#198440PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW89FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro W61*F
Plasmid#198435PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW61*FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
eZiPro W50F
Plasmid#198437PurposeActivity/inhibition assays, 19F-NMR assignmentDepositorInsertenzymatically cleavable linked Zika virus NS2B-NS3
UseTagsHis6-TEVExpressionBacterialMutationW50FPromoterT7 promoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac1-Nucleocapsid
Plasmid#179696PurposeExpresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cellsDepositorInsertSARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
UseTagsHis6-TEV protease siteExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJKW 0643
Plasmid#74121PurposePhotinus pyralis luciferin sulfotransferase LST - pHis8-4DepositorInsertPhotinus pyralis firefly luciferin sulfotransferase
UseTags8x-His, TEV protease site (ENLYFQ)ExpressionBacterialMutationPromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only