Showing: 381 - 400 of 1392 results
-
Plasmid#61271PurposeAlso referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Eef1a-1955/-1>nls::Cas9::nls
Plasmid#59987PurposeEef1a (EF1alpha) promoter driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRTagsExpressionMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Eef1a (EF1alpha) -1955/-1AvailabilityAcademic Institutions and Nonprofits only -
Mesp-1916/-1>nls::Cas9::nls
Plasmid#59988PurposeMesp driver driving nls::Cas9::nlsDepositorInsertnls::Cas9::nls
UseCRISPRTagsExpressionMutationReverted mutations from dCas9 to wiltdtypePromoterCiinte.Mesp -1916/-1AvailabilityAcademic Institutions and Nonprofits only -
pMJ806
Plasmid#39312DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMJ825
Plasmid#39315DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationAspartate 10 to Alanine (D10A)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMJ826
Plasmid#39316DepositorInsertCas9
UseCRISPRTagsHis6 and MBPExpressionBacterialMutationHistidine 840 to Alanine (H840A)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pSL690
Plasmid#47753PurposeExpresses dCas9-VP64 fusionDepositorInsertdCas9-VP64
UseCRISPRTags3x FLAG, NLS, and VP64ExpressionMammalianMutationD10A and H840A mutations in Cas9PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pKB01_mCherry
Plasmid#69104PurposeExpresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBSKΔB-CBh-Cas9n-pA
Plasmid#82617PurposeExpresses Cas9 nickase in mammalian cellsDepositorInsertSpCas9n
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pScI_dCas9-CDA-UL
Plasmid#108551PurposeBacterial Target-AID UGI-LVA vectorDepositorInsertdCas9-PmCDA1-UGI-LVA
UseTagsFlagExpressionBacterialMutationD10A and H840A for SpCas9Promoterlambda ORAvailabilityAcademic Institutions and Nonprofits only -
pFD115
Plasmid#124768PurposepFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorInsertsPtet
cas9
term PpflB sgRNA (BsaI)
UseCRISPRTagsExpressionMutationPromoterPtetAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-Dnmt3a-Dnmt3l-P2A-eGFP
Plasmid#128424PurposeHuman Codon Optimized dCas9-DNMT3A3LDepositorInsertdCas9m4-sv40NLS-DNMT3A-3L-sv40NLS-P2A-eGFP
UseCRISPR and LentiviralTagsGFPExpressionMammalianMutationPromoterT7 PromoterAvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0017
Plasmid#117527PurposeLevel0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)DepositorInsertSp-eCas9 1.0 (no stop codon)
UseCRISPRTagsExpressionMutationK810A, K1003A, R1060APromoterAvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0019
Plasmid#117529PurposeLevel0 Golden Gate part: CDS, Sp-eCas9 1.1 (no stop codon)DepositorInsertSp-eCas9 1.1 (no stop codon)
UseCRISPRTagsExpressionMutationK848A, K1003A, R1060APromoterAvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0011
Plasmid#117530PurposeLevel0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)DepositorInsertSp-xCas9 3.7 (no stop codon)
UseCRISPRTagsExpressionMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCas9-J23113
Plasmid#119701PurposeExpression of Cas9 with a weak promoterDepositorInsertCas9
UseCRISPRTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pICH47742:PtFCP:Cas9YFP
Plasmid#104894PurposeCas9, with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformationDepositorInsertCas9
UseCRISPR; ; golden gate assemblyTagsYFPExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pX165-Sniper-Cas9
Plasmid#140560PurposeExpresses Sniper Cas9 in mammalian cellsDepositorInsertCas9
UseTagsExpressionMutationF539S M763I K890NPromoterAvailabilityAcademic Institutions and Nonprofits only -
pX165-LZ3 Cas9
Plasmid#140561PurposeExpresses LZ3 Cas9 in mammalian cellsDepositorInsertCas9
UseTagsExpressionMutationN690C T769I G915M N980KPromoterAvailabilityAcademic Institutions and Nonprofits only -
pX165-Cas9-HF1
Plasmid#140565PurposeExpresses Cas9-HF1 in mammalian cellsDepositorInsertCas9
UseTagsExpressionMutationN497A R661A Q695A Q926APromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 381 - 400 of 1392 results