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Plasmid#193302PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the nucleusDepositorInsertG6PD (G6PD Human)
UseTagsSV40 NLS and mTurqoise2ExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available sinceDec. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A
Plasmid#111455PurposeTransient expression of Flag-tagged human Sox9 with T236A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT236A mutationPromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDO001
Plasmid#198828PurposeInhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.DepositorInsert15xUAS::miniSOG-103L-VAMP2-mCherry::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T240A
Plasmid#111457PurposeTransient expression of Flag-tagged human Sox9 with T240A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT240APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-GLI1
Plasmid#79497PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human GLI1 CDSDepositorInsertGLI1 (GLI1 Human)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2-Sox9 T236A/T240A
Plasmid#111458PurposeTransient expression of Flag-tagged human Sox9 with T236A/T240A mutationDepositorInsertSox9 (SOX9 Human)
UseTagsFlagExpressionMammalianMutationT236A and T240APromoterCMVAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG69 G6PD 3'UTR mut
Plasmid#12053DepositorInsertG6PD 3'UTR mut (G6PD Human)
UseLuciferaseTagsExpressionMammalianMutationMutations in microRNA recognition sites (seed mat…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-pegRNA_HOXD13_CtoT-EGFP
Plasmid#159792PurposeS. pyogenes pegRNA for C to T mutation at the HOXD13 site of mouse cells using prime editingDepositorInsertspacer of pegRNA_HOXD13_CtoT targeting HOXD13 gene (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-pegRNA_HOXD13_CtoG-EGFP
Plasmid#159791PurposeS. pyogenes pegRNA for C to G mutation at the HOXD13 site of mouse cells using prime editingDepositorInsertspacer of pegRNA_HOXD13_CtoG targeting HOXD13 gene (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHW582
Plasmid#198819PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, higher baseline fluorescenceDepositorInsert15xUAS::GCaMP7b-SL2-mKate2::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR012
Plasmid#198823PurposeNucleolus-transport-enhanced Cre protein. Recombination-mediated gene expression knockout.DepositorInsert15xUAS::nCRE-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW539
Plasmid#198811PurposeDominant negative form of Protein Kinase A (PKA). Lowers PKA activity in targeted neurons.DepositorInsert15xUAS::PKA(DN)-SL2-GFP::let-858 3'UTR
UseTagsExpressionWormMutationG310D mutation to abolish the binding of cAMP of …PromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD002
Plasmid#198833PurposeInhibition of synaptic vessel release due to disruption of synaptic vessel function by reactive oxygen species production.DepositorInsert15xUAS::miniSOG-103L-mCherry::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHW580
Plasmid#198816PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, slow kineticsDepositorInsert15xUAS::GCaMP7s-SL2-mKate2::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Perox-mTurq2-Apollo-NADP+
Plasmid#193304PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the peroxisomeDepositorInsertG6PD (G6PD Human)
UseTagsmTurqoise2, peroxisomal targeting sequenceExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
alpha 0-CFP in pdest12.2
Plasmid#36312DepositorInsertG protein alpha 0 (Gnao1 Rat)
UseTagsCFPExpressionMammalianMutationIntroduced KpnI and SpeI sites between 92nd and 9…PromoterCMVAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSOX9-dTAG-mNeonGreen-V5
Plasmid#194971PurposeAAV vector for knocking in a C-terminal tag (dTAG-mNeonGreen-V5) for human SOX9DepositorInsertSRY-box transcription factor 9 (SOX9 Human)
UseAAVTagsdTAG-mNeonGreen-V5ExpressionMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET-45b-HOXD13-IDR-mCherry
Plasmid#145280PurposeBacterial Expression of 6xHis HOXD13 IDR mCherry Fusion ProteinDepositorInsertHOXD13-IDR (HOXD13 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13-IDR-mCherry-CRY2
Plasmid#145258PurposeMammalian Expression of Opto HOXD13 IDRDepositorInsertHOXD13wt-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only