Addgene

We narrowed to 2,130 results for: CO

Showing: 381 - 400 of 2130 results

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pDEST47-SARA-GFP

Plasmid

#67410

Purpose

Mammalian expression of hSara with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-mTurquoise2-NLS

Plasmid

#165443

Purpose

Expresses nuclear mTurquoise2 from CMV promoter

Depositor

Esteban Engel

Article

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

mTurquoise2-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a

Plasmid

#216385

Purpose

Baculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)

Depositor

William Weis

Article

Bruguera et al bioRxiv 2024.02.03.578714

Insert

Norrin (NDP Human)

Use

Baculovirus

Tags

GP64 signal sequence-MBP-3C and Rho1D4

Expression

Insect

Mutation

C93A/C95A/C131A

Promoter

Polyhedrin

Available since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEntr-Mef2aflbio

Plasmid

#32970

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

Mef2a (MEF2A Human)

Use

Gateway

Tags

flag-bio

Expression

Mutation

Promoter

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA-3xflagGREB1a

Plasmid

#112221

Purpose

mammalian expression of flag tagged GREB1a

Depositor

Craig Burd

Article

Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

Insert

GREB1a (GREB1 Human)

Use

Tags

3x flag

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMC-1-AtUbi10-6

Plasmid

#176871

Purpose

MoClo Level 0 plasmid, contains AtUbi1 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

AtUbi10

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAL612

Plasmid

#119860

Purpose

precusor outer membrane protein A, ompA, with mutations Cysteine 290 Serine, Glycine 244 Cysteine

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

outer membrane protein A, ompA, with mutations Cysterine 290 to Serine and Glycine 244 to Cysteine

Use

Tags

none

Expression

Bacterial

Mutation

Cysterine 290 to Serine and Glycine 244 to Cystei…

Promoter

lac

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ASPM CTR

Plasmid

#27669

Depositor

Joseph Loturco

Article

Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

Insert

Abnormal spindle-like microcephaly associated (Aspm Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Contains carboxy terminal region of ASPM (amino a…

Promoter

Available since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pAL725

Plasmid

#119862

Purpose

precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)

Use

Tags

none

Expression

Bacterial

Mutation

Leucine 244 to Cysteine, Aspartate 287 to Cysteine

Promoter

T5

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBR322-bom

Plasmid

#155178

Purpose

pBICI_2_strong without bom site

Depositor

Bernd Nidetzky

Article

Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

Inserts

Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
Cellobiose phosphorylase from Cellulomonas uda

Use

Synthetic Biology

Tags

His Tag and Strep - Tag II

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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sm-9xGCN4-Renilla-MS2

Plasmid

#178009

Purpose

Expresses Spaghetti Monster (sm) 9xGCN4-Renilla mRNA reporter in mammalian cells

Depositor

Jeffrey Chao

Article

Gomez-Puerta et al Elife. 2022 Jun 22;11. pii: 75580. doi:

Insert

sm-9xGCN4

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-3xflagGREB1b

Plasmid

#112222

Purpose

mammalian expression of flag tagged GREB1b

Depositor

Craig Burd

Article

Haines et al Endocr Relat Cancer. 2018 Jul;25(7):735-

Insert

GREB1b (GREB1 Human)

Use

Tags

3x flag

Expression

Mammalian

Mutation

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pminiT-MED6-Halo donor

Plasmid

#197866

Purpose

Donor plasmid used for building MED6-Halo knock-in mESCs

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

Med6 (Med6 Mouse)

Use

Mouse Targeting

Tags

Halotag

Expression

Mutation

Promoter

NA

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pX330_ATP1A1_G7

Plasmid

#173204

Purpose

Expresses the ATP1A1 G7 sgRNA in combination with SpCas9 to target ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + SpCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-SARA-V5-His6

Plasmid

#67431

Purpose

Expression of hSara with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
M-R742X (BF7-2)

Plasmid

#21372

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type II (PKD2 Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

changed R742 to stop codon

Promoter

Available since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pET-DEST42-SAR1-V5-His6

Plasmid

#67430

Purpose

Expression of hSar1 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SAR1 (SAR1A Human)

Use

Tags

His and V5

Expression

Bacterial

Mutation

Promoter

T7/lacO

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAJ01

Plasmid

#14908

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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mP14H1

Plasmid

#84369

Purpose

antigen for mouse PRDM14 purification ( aa 1-231)

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14

Use

Tags

6xHis

Expression

Bacterial

Mutation

only aa 1-231 present

Promoter

T7

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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hP14H8y

Plasmid

#84368

Purpose

antigen for human PRDM14 purification ( aa 1-243)

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

contains PRDM14 AA1-243

Promoter

T7

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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