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Showing: 21 - 40 of 6946 results

  4. pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP

    Plasmid
    #52053
    Purpose
    Used in phosphoprotein synthesis. Replace the sfGFP with gene of interest (GOI). Add TAG amber codon(s) at the position(s) in GOI where phosphoserine incorporation is desired.
    Depositor
    Jesse Rinehart
    Insert
    sfGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tetR/pLtetO
    Available since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    52053-plasmid-map-sequence-id-442992

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  6. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

    Plasmid
    #53225
    Purpose
    human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis
    Depositor
    Jesse Rinehart
    Insert
    MEK1 (MAP2K1 Human)
    Use
    Phosphoprotein synthesis
    Tags
    HIS and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli, both codons for Ser21…
    Promoter
    pLtetO
    Available since
    May 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    53225-plasmid-map-sequence-id-82901

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  7. pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid
    #116955
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    116955-plasmid-map-sequence-id-226973

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  8. pBEST-PLtetO-1-UTR1-TetR-deGFP-T500

    Plasmid
    #45774
    Depositor
    Richard Murray
    Insert
    tetR-deGFP
    Use
    Synthetic Biology
    Tags
    Fusion protein of TetR and deGFP, with glycine-se…
    Expression
    Bacterial
    Mutation
    Promoter
    PLtetO-1
    Available since
    July 19, 2013
    Availability
    Academic Institutions and Nonprofits only
    45774-plasmid-map-sequence-id-64148

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  9. pFS_0272_pET30_pCat-tetR-Term-ptetA-Rluc-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

    Plasmid
    #116956
    Purpose
    Expresses FsRT-Cas1-Cas2 under pT7lac and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout
    Depositor
    Randall Platt
    Article
    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:
    Insert
    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    116956-plasmid-map-sequence-id-227069

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  16. pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

    Plasmid
    #70195
    Purpose
    Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector
    Depositor
    Richard Murray
    Article
    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.
    Insert
    tetR-deGFP fusion
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    70195-plasmid-map-sequence-id-125453

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  19. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Ronald Davis
    Article
    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    73796-plasmid-map-sequence-id-237125

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  20. pLenti-Tet

    Plasmid
    #138086
    Purpose
    Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles
    Depositor
    Eva Frickel
    Article
    Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    minimal CMV promoter with heptamerized tet‐operat…
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    138086-plasmid-map-sequence-id-276295

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Showing: 21 - 40 of 6946 results