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Plasmid#220625PurposeSource for TEF1 MS2 coat fusion protein fusionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only
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BB1_12_pTEF2
Plasmid#98507PurposeBB1 with insert: promoter PTEF2DepositorInsertTEF2 promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p414TEF_Hsp82_noTER
Plasmid#61714Purposeyeast plasmid with Hsp82DepositorInsert6xHis-Hsp82 (HSP82 Budding Yeast)
UseTags6xHisExpressionYeastMutationPromoterTEF1Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pTEF_Cas9
Plasmid#104909PurposehCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selectionDepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPL5071_TEF1*-Cre_URA3
Plasmid#60930PurposeConstitutive expression vector for Cre-recombinase containing URA3 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-Sir2
Plasmid#51742Purposeoverexpression of Sir2 in S. cerevisiae, HIS3 markerDepositorInsertSir2 (SIR2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPL5628_TEF1*-Cre_MET15
Plasmid#60933PurposeConstitutive expression vector for Cre-recombinase containing MET15 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPL5608_TEF1*-Cre_TRP1
Plasmid#60931PurposeConstitutive expression vector for Cre-recombinase containing TRP1 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromoterMutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
LT1_33_pTEFmut8-mCherry
Plasmid#177706PurposeConstruct for genome integration expressing constitutively mCherry under pTEFmut8 promoterDepositorInsertpTEFmut8-mCherry
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_StefinA
Plasmid#182402PurposeBacterial expression of stefinADepositorInsertstefin (CSTA Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-PKM1
Plasmid#34607DepositorInsertPKM1 (PKM Human)
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-PKM2
Plasmid#34608DepositorInsertPKM2 (PKM Human)
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUPD-pTEF2 (01-11)
Plasmid#101685PurposepTEF2 in domestication vectorDepositorInsertGGAG-[promoter of S. cerevisiae YBR118W (520bp)]-CCAT
UseYeast expressionTagsExpressionMutationPromoterAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPL5606_TEF1*-Cre_ADE2
Plasmid#60932PurposeConstitutive expression vector for Cre-recombinase containing ADE2 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSNR52-sgTEF1
Plasmid#46922PurposeYeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoterDepositorInsertsgTEF1 promoter (TEF1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-SirT1
Plasmid#51746Purposeoverexpression of human SirT1 in S. cerevisiae, HIS3 markerDepositorInsertSirT1 (SIRT1 Human)
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET24c(+)-mtEFTu
Plasmid#30535DepositorInsertMature bovine mitochondrial elongation factor Tu
UseTagsHisExpressionBacterialMutationMature form amino acids 44-452. Mito import seque…PromoterAvailable sinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTEF-Sup35C
Plasmid#1202DepositorInsertSup35 C-terminus (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationSup35 C-terminus ~254aa-endPromoterTEFAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
p416TEF-SEC16
Plasmid#102676PurposeThe gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretionDepositorInsertSEC16 (SEC16 Budding Yeast)
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
P413TEF-PYK1
Plasmid#34605DepositorInsertPYK1
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only