Addgene

We narrowed to 15,747 results for: puromycin

Showing: 21 - 40 of 15747 results

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FASTHDR-Cterm-mClover3-Puromycin

Plasmid

#167205

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mClover3 and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

mClover3

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE-T2-∆mIR-IRES-Puromycin

Plasmid

#105168

Purpose

Mammalian expression vector for expressing tandem cDNAs

Depositor

Michael E. Wright

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tet/CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-NanoLuc-Puromycin

Plasmid

#167209

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

NanoLuc

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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plko-puromycin-shTRX1-259

Plasmid

#110921

Purpose

To knockdown Thioredoxin-1

Depositor

Priyamvada Rai

Article

Young et al Biochem Biophys Res Commun. 2010 Feb 12;

Insert

TRX1 (TXN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

unknown

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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plko-tet-on puromycin-shMTH1

Plasmid

#110918

Purpose

To knock down MTH1 (Mut T Homolog 1) after addition of doxycline

Depositor

Priyamvada Rai

Article

Patel et al Oncogene. 2015 May 14;34(20):2586-96. do

Insert

MTH1 (NUDT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-CCO-IRES-Puromycin

Plasmid

#134861

Purpose

Expresses EGFP-tagged inner mitochondrial membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

cytochrome c oxidase subunit VIII (COX8A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-PTS1-IRES-Puromycin

Plasmid

#134665

Purpose

Expresses EGFP-tagged peroxisome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

tripeptide SKL (Peroxisome targeting signal 1)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-GalT-IRES-Puromycin

Plasmid

#134862

Purpose

Expresses EGFP-tagged Golgi marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

beta 1,4-galactosyltransferase (B4GALT1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 83-398

Promoter

EF1a

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-PTDSS1-IRES-Puromycin

Plasmid

#134667

Purpose

Expresses EGFP-tagged Mitochondria-associated ER membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

PTDSS1 (PTDSS1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-Neuromodulin-IRES-Puromycin

Plasmid

#134666

Purpose

Expresses EGFP-tagged plasma membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Neuromodulin (GAP43 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-G1-ATP6-Myc-FLAG (puromycin)

Plasmid

#86851

Purpose

mammalian expression of ATP6 with mitochondrial targeting sequence, Myc, and FLAG tags

Depositor

Matthew O'Connor

Article

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP6 (ATP6 Human)

Use

Tags

ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

Expression

Mammalian

Mutation

codon corrected

Promoter

CMV

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

Plasmid

#115519

Purpose

To construct multiple sgRNA expression vector for CRISPR

Depositor

Xingxu Huang

Insert

ccdB-sgRNA scaffold

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK-puromycin-AflII

Plasmid

#106404

Purpose

Empty guide RNA cloning vector with an AflII site added from Addgene 41824

Depositor

Jason Gertz

Article

Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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plko-tet-on-puromycin-shTRX1-259

Plasmid

#110920

Purpose

To knockdown Thioredoxin-1 following doxycycline addition

Depositor

Priyamvada Rai

Article

Samaranayake et al Nat Commun. 2017 Oct 31;8(1):1204. doi:

Insert

TRX1 (TXN Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

unknown

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB7A-IRES-Puromycin

Plasmid

#133027

Purpose

Expresses EGFP-tagged late endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAB7A (RAB7A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-Calr-KDEL-IRES-Puromycin

Plasmid

#134664

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

calreticulin (CALR Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 18-413

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

Plasmid

#133028

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Golgin84 (GOLGA5 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-673

Promoter

Human elongation factor 1 alpha

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

Plasmid

#134868

Purpose

Expresses EGFP-tagged lysosomal marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lysosomal-associated membrane protein 1 (LAMP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin

Plasmid

#134858

Purpose

Expresses EGFP-tagged early endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Ras-related protein 5A

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAB11B-IRES-Puromycin

Plasmid

#134860

Purpose

Expresses EGFP-tagged recycling endosome marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAB11B (RAB11B Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 21 - 40 of 15747 results

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