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Plasmid#165786PurposeDrosophila Polyubiquitin promoter, strongest promoter in the libraryDepositorInsertPolyUbiq
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pssAAV_Seq17_CMV_bglobin_3NLS_RFP_WPRE_polyA
Plasmid#220222PurposeAAV-STARR-seq validation vector with RFPDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pssAAV_Seq17_CMV_bglobin_3NLS_GFP_WPRE_polyA
Plasmid#220221PurposeAAV-STARR-seq validation vector with GFPDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
polySUMO1
Plasmid#221066PurposeExpression of polySUMO1 protein under T7 promoterDepositorInsertFive repeats of SUMO1 each separated by (GGS)4 (SUMO1 Human)
UseTagsHis and MBPExpressionBacterialMutationPromotertacAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
polySIM
Plasmid#221068PurposeExpression of polySIM protein under T7 promoterDepositorInsert10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4
UseTagsHis and MBPExpressionBacterialMutationPromoterunknownAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
polySUMO3
Plasmid#221067PurposeExpression of polySUMO3 protein under T7 promoterDepositorInsertFive repeats of SUMO3 each separated by (GGS)4 (SUMO3 Human)
UseTagsHis and MBPExpressionBacterialMutationPromotertacAvailable sinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
LvPolyUb
Plasmid#218982PurposeL.variegatus polyubiquitin-C promoterDepositorInsertL.variegatus polyubiquitinC promoter
UseSea urchinTagsExpressionMutationPromoterL.variegatus polyubiquitinC promoterAvailable sinceAug. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pssAAV_Seq17_CMV_bglobin_3NLS_TFP_WPRE_polyA
Plasmid#220223PurposeAAV-STARR-seq validation control vector with TFPDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Seq17_VS33_4.26_ChimeraIntron_3NLS_RFP_WPRE_polyA
Plasmid#220225PurposeAAV-STARR-seq validation vector containing VS33 enhancerDepositorInsertEnhancer (VS33)
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
NfPolyA_pET21b
Plasmid#215413PurposeExpression construct for NfPolyA gene, codon optimized for expression in E. coliDepositorInsertNfPolyA
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOLY_new
Plasmid#179276PurposeExpresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)DepositorInsertscellodextrin phosphorylase
cellobiose phosphorylase
sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)
UseSynthetic BiologyTags6xHIS and Strep-Tag IIExpressionBacterialMutationPromoterT7 lacOAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLz54_polyPro_polar
Plasmid#140847PurposeSame as pLz47 but with 20 polar amino acids instead of N*- peptideDepositorInsertpolyproline helix with polar residues
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz58_polyPro_CsoS2_CTP
Plasmid#140850PurposeSame as pLz47 but with C-terminal peptide (CTP) instead of N*- peptideDepositorInsertpolyproline helix with CsoS2 C-terminal peptide
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz55_polyPro_random_Npeptide
Plasmid#140846PurposeSame as pLz47 but with randomized N*-peptide sequenceDepositorInsertpolyproline helix with randomized CsoS2 N-peptide
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz56_polyPro_CsoS2_MR_r1
Plasmid#140848PurposeSame as pLz47 but with MR repeat 1 instead of N*-peptideDepositorInsertpolyproline helix with CsoS2 MR repeat 1
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz47_polyPro_CsoS2_Npeptide
Plasmid#140845PurposeConsensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backboneDepositorInsertpolyproline helix with CsoS2 N-peptide
UseTagsHexahistidine tag and Strep-tag IIExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLz57_polyPro_CsoS2_CTD_r8
Plasmid#140849PurposeSame as pLz47 but with CTD repeat 8 instead of N*-peptideDepositorInsertpolyproline helix with CsoS2 CTD repeat 8
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_polio_3ABC
Plasmid#212986PurposeEncodes Poliovirus type I Mahoney 3ABC optimized for expression in mammalian transcription/translation extractsDepositorInsertPoliovirus Type I Mahoney 3ABC
UseMammalian cell free protein expressionTagsHAExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOliSpec5'
Plasmid#228551PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmidDepositorInsertTruncated 5' region of aadA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOliSpec3'
Plasmid#228550PurposeTemplate for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.DepositorInsertTruncated 3' region of aadA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only