Addgene

We narrowed to 28,243 results for: pET

Showing: 21 - 40 of 28243 results

Results per page:

Sort results by:

pET-3a_mb2m

Plasmid

#211368

Purpose

Expresses murine b2m

Depositor

Adnane Achour

Insert

b2m (B2m Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-21a_3xNLS_SpCas9_protein_expression

Plasmid

#114365

Purpose

Protein expression plasmid for 3xNLS SpCas9 in pET-21a backbone

Depositor

Scot Wolfe

Article

Wu et al Nat Med. 2019 May;25(5):776-783. doi: 10

Insert

3xNLS_SpCas9

Use

CRISPR

Tags

NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-NtC60αESNtLC60β

Plasmid

#160878

Purpose

Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli.

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, groES

Use

Tags

Expression

Bacterial

Mutation

Promoter

rbcL: T7, chaperones: T7

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-NtLNtC60αESC60β

Plasmid

#160875

Purpose

Expression of N. tabacum Rubisco large subunit (rbcL), Rubisco chaperone protiens cpn60α, cpn60β, and E. coli groES in E. coli

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, groES

Use

Tags

Expression

Bacterial

Mutation

Promoter

rbcL: pBAD, chaperones: T7

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pet-MBP

Plasmid

#209184

Purpose

Expression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coli

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Type

Empty backbone

Use

Tags

HisX6

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-GST

Plasmid

#42049

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

GST

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-11b_PcrA_M6_H93A

Plasmid

#194156

Purpose

Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

Depositor

Taekjip Ha

Article

Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

Insert

Engineered helicase PcrA_M6

Use

Tags

6x His tag and FLAG tag

Expression

Bacterial

Mutation

H93A, C96A, N187C, C247A, L384V, L409C

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-BiFC

Plasmid

#87856

Purpose

pETDUET-1 based vector for use in BiMolecular Fluorescence Complementation based assays. MCS at 5' of each BiFC fragment.

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Inserts

Encodes for MCS & C-term half of mVenus
Encodes for MCS & N-term half of mVenus

Use

Tags

BiFC C-terminal fragment

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-mKelly2

Plasmid

#119783

Purpose

Expresses mKelly2 tagged N-terminally with an MHHHHHHGSG tag

Depositor

Stephen Mayo

Article

Wannier et al Proc Natl Acad Sci U S A. 2018 Nov 27;11

Insert

mKelly2

Use

Tags

6xHis tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-CjCas9

Plasmid

#89754

Purpose

Expression of CjCas9 with His tag in E.coli

Depositor

Seokjoong Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

CjCas9

Use

CRISPR

Tags

6XHis, HA, and SV40 NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-15b_LC3B

Plasmid

#73949

Purpose

For recombinant expression of human LC3B/MAP1LC3B in E. coli

Depositor

Dieter Willbold

Article

Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

Insert

LC3B (MAP1LC3B Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only
pET-MAT2A

Plasmid

#164820

Purpose

Expresses MAT2A in E. coli BL21(DE3)

Depositor

Vern Schramm

Article

Firestone et al J Am Chem Soc. 2017 Oct 4;139(39):13754-

Insert

Methionine Adenosyltransferase 2A (MAT2A Human)

Use

Tags

6x His Tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-SMAD4

Plasmid

#16491

Depositor

Bert Vogelstein

Article

Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

Insert

Smad4 (SMAD4 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pET-28GG

Plasmid

#104655

Purpose

Level 2 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-Neq2X

Plasmid

#182365

Purpose

Expresses Neq2X DNA polymerase

Depositor

Morten Norholm

Article

Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

Insert

Neq2X

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-TRSter

Plasmid

#78865

Purpose

Expresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS element

Depositor

Elias Arnér

Article

Arner et al J Mol Biol. 1999 Oct 8;292(5):1003-16.

Insert

Thioredoxin Reductase (Txnrd1 Rat)

Use

Tags

Mutated E. coli-type SECIS element (3’ end of ins…

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-PesaRlux

Plasmid

#47802

Purpose

PesaR promoter, luxCDABE, and terminator in pET17b (AmpR)

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

PesaR-luxCDABE-term

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PesaR

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pET-AspS

Plasmid

#159618

Purpose

Expression plasmid for AcpS phosphopantetheinyl transferase

Depositor

Christopher T Walsh, Jun Yin

Article

Zhou et al ACS Chem Biol. 2007 May 22;2(5):337-46.

Insert

AcpS

Use

Tags

6HIS

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-NtLNtC60αC20C60β

Plasmid

#160874

Purpose

Expression of N. tabacum Rubisco large subunit (rbcL) and Rubisco chaperone protiens cpn60α, cpn60β, cpn20 in E. coli

Depositor

Maureen Hanson

Article

Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

Insert

rbcL, cpn60α, cpn60β, cpn20

Use

Tags

Expression

Bacterial

Mutation

Promoter

rbcL: pBAD, chaperones: T7

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-S9

Plasmid

#132948

Purpose

Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S9 (rpsI E.coli)

Use

Tags

N-terminal 6xHis-tag

Expression

Bacterial

Mutation

E52C

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge