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Plasmid#203748PurposeEncodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.DepositorInserttrLAToC27AC30A (Lat Mouse)
UseTagsER translocation signal - mEos3.2ExpressionMammalianMutationC27A, C30APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
p214_LTJ_1kbCD90.1Template
Plasmid#82667Purpose1kb HDR template for murine CD90.1DepositorInsert1kb genomic DNA sequence from murine CD90.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p229_LTJ_2kbCD90.1Template
Plasmid#82668Purpose2kb HDR template for murine CD90.1DepositorInsert2kb genomic DNA sequence from murine CD90.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p213_LTJ_160bpCD90.1Template
Plasmid#82666Purpose160bp HDR template for murine CD90.1DepositorInsert160bp genomic DNA sequence from murine CD90.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p230_LTJ_4kbCD90.1Template
Plasmid#82669Purpose4kb HDR template for murine CD90.1DepositorInsert4kb genomic DNA sequence from murine CD90.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PLAT_Trypsin
Plasmid#110039PurposeProtein expression and purification of PLAT_TrypsinDepositorInsertPLAT_Trypsin (PLAT Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
p248_LTJ_2kbCD45.1Template
Plasmid#82662Purpose2kb HDR template for murine CD45.1DepositorInsert2kb genomic DNA sequence from murine CD45.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_Malat1_D
Plasmid#72623PurposeExpresses two gRNAs targeting MALAT1 promoterDepositorInsertgRNAs toward Malat1
UseTagsExpressionMammalianMutationPromoterU6 (gRNA1) and H1 (gRNA2)Available sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CLTC_Clathrin
Plasmid#109997PurposeProtein expression and purification of CLTC_ClathrinDepositorInsertCLTC_Clathrin (CLTC Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_Malat1_E
Plasmid#72624PurposeExpresses two gRNAs targeting MALAT1 promoterDepositorInsertgRNAs toward Malat1
UseTagsExpressionMammalianMutationPromoterU6 (gRNA1) and H1 (gRNA2)Available sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
p242_LTJ_1kbCD45.1Template
Plasmid#82661Purpose1kb HDR template for murine CD45.1DepositorInsert1kb genomic DNA sequence from murine CD45.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
BlatCas9
Plasmid#163792PurposeThe plasmid expresses human codon-optimized BlatCas9 and gRNA expression elements.DepositorTypeEmpty backboneUseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
BB1_12_pLAT1
Plasmid#98509PurposeBB1 with insert: promoter PLAT1DepositorInsertLAT1 promoter
UseTagsExpressionBacterialMutationInternal BsaI and BpiI sites removed from insertPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p350_LTJ_180bpCD90.1Template
Plasmid#104988Purpose180bp HDR template for murine CD90.1DepositorInsert180bp genomic DNA sequence from murine CD90.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
insulator_seq_barcode_vector
Plasmid#221458PurposeVector for generating a barcode library for insulator-seq.DepositorTypeEmpty backboneUseSynthetic Biology and UnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
insulator_seq_UENU_donor
Plasmid#221459PurposeVector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.DepositorInsert3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
insulator_seq_reporter_vector
Plasmid#221460PurposeVector containing eGFP and mCherry reporter genes, for assembling an insulator-seq reporter library.DepositorInsertgypsy insulator-EGFP-neutral spacer DNA-mCherry
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_LCLAT1_GREB10
Plasmid#205845PurposeExpress mEGFP-tagged fusion protein, LCLAT1_GREB1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLAT-P
Plasmid#203747PurposeEncodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. The palmitoylation site at C27 is mutated to A. Used as a fluorescent plasma membrane probe.DepositorInserttrLAToC27A (Lat Mouse)
UseTagsER translocation signal - mEos3.2ExpressionMammalianMutationC27APromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLATo
Plasmid#203746PurposeEncodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.DepositorInserttrLATo (Lat Mouse)
UseTagsER translocation signal - mEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only