Addgene

We narrowed to 759 results for: golden gate assembly

Showing: 21 - 40 of 759 results

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pET-Gate2 mazEF

Plasmid

#117117

Purpose

Destination vector with mazEF toxin antitoxin system

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Type

Empty backbone

Use

Backbone for golden gate assembly for gene target…

Tags

Expression

Mutation

Promoter

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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p370_DG

Plasmid

#234693

Purpose

Golden gate domesticated pET28b with 3C-mNeongreen-His10

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

3C-mNeongreen-His10

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p277_DG

Plasmid

#234695

Purpose

Golden gate domesticated pET28b for scarless cloning with His6-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His6-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p357_DG

Plasmid

#234700

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-GST-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-GST-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p349_DG

Plasmid

#234679

Purpose

Golden gate domesticated pET28b with His10-GST-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-GST-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p351_DG

Plasmid

#234681

Purpose

Golden gate domesticated pET28b with His10-mNeongreen-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-mNeongreen-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p364_DG

Plasmid

#234686

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and Opsin

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-bdNEDD8 and Opsin

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p354_DG

Plasmid

#234697

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-TEV

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-TEV

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p358_DG

Plasmid

#234701

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-bdNEDD8

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-bdNEDD8

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p359_DG

Plasmid

#234702

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-mNeongreen-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-mNeongreen-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p360_DG

Plasmid

#234703

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-mNeongreen-bdNEDD8

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-mNeongreen-bdNEDD8

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p348_DG

Plasmid

#234678

Purpose

Golden gate domesticated pET28b with His10-MBP-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-MBP-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p356_DG

Plasmid

#234699

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-MBP-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-MBP-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p355_DG

Plasmid

#234698

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-Thrombin

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-Thrombin

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p352_DG

Plasmid

#234682

Purpose

Golden gate domesticated pET28b with His10-mNeongreen-bdNEDD8

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-mNeongreen-bdNEDD8

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p363_DG

Plasmid

#234685

Purpose

Golden gate domesticated pET28b with His10-bdNEDD8 and Sortase

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-bdNEDD8 and Sortase

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p353_DG

Plasmid

#234696

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-3C

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His10-3C

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pOGG004

Plasmid

#113979

Purpose

Vector construction module. Level 1 golden gate cloning site module with LacZ reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Level 1 golden gate cloning site

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pOGG006

Plasmid

#113981

Purpose

Golden Gate Level 2 cloning site, position 1 module for vector construction

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Level 2 golden gate cloning vector

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p361_DG

Plasmid

#234683

Purpose

Golden gate domesticated pET28b with His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

Depositor

Matthew Wohlever

Article

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Use

Tags

His6-3C-3XFLAG-SUMO and Thrombin-HiBiT

Expression

Bacterial

Mutation

No

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge