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Plasmid#50024PurposepENTR vector for mouse Crk-IIDepositorInsertCrk-II (Crk Mouse)
UseEntry vectorTagsExpressionMutationPromoterAvailable sinceDec. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTU2-b_CrK_amilCP
Plasmid#204067PurposeType TU2-b backbone with kanamycin resistance and ColE1-bom-rop ORI. amilCP dropout vector.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A_CrK_amilCP
Plasmid#204046PurposeType TU1-A backbone with kanamycin resistance and ColE1-bom-rop ORI. amilCP dropout vector.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationNonePromoterAvailable sinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzl_CRKL-W275L
Plasmid#196019PurposeExpresses CRKL W275LDepositorInsertCRKL
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3_CRKL-silent-V5
Plasmid#196015PurposeExpresses CRKL-silent, V5 taggedDepositorInsertCRKL
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3_CRKL-W160L-V5
Plasmid#196017PurposeExpresses CRKL W160L, V5 taggedDepositorInsertCRKL
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
plx304_CRKL-open-V5
Plasmid#196023PurposeExpresses CRKL open, V5 taggedDepositorInsertCRKL
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-chiRNA_dCrkgRNA1
Plasmid#131139PurposeEncodes gRNA targeting Drosophila crk locus. Cuts 110bp upstream of the transcription start site. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).DepositorInsertdCrk gRNA1 (Crk Fly)
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-chiRNA_dCrkgRNA2
Plasmid#131140PurposeEncodes gRNA targeting Drosophila crk locus. Cuts 99bp beyond the 3' UTR. gRNA cloned into BbsI site of pU6-BbsI-chiRNA (Gratz et al. Genetics. 2013).DepositorInsertdCrk gRNA1 (Crk Fly)
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
CRK7A-c021
Plasmid#53643PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertCRK7A
UseBaculovirus expressionTagsHis6-TEVExpressionMutationPromoterpolyhedrinAvailable sinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSG5-CRKL-Y198FY207F
Plasmid#32506DepositorInsertCRKL (CRKL Human)
UseTagsExpressionMammalianMutationY207F and Y198FPromoterAvailable sinceJan. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX CrkL-SH2
Plasmid#46419DepositorInsertCrkl (CRKL Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 9-108PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTIGER_mNeonGreen::3xFLAG::dCrk
Plasmid#131137PurposeUASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorInsertCrk (Crk Fly)
UseTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTIGER_tdTomato::3xFLAG::dCrk
Plasmid#131138PurposeUASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.DepositorInsertCrk (Crk Fly)
UseTags3XFLAG and tdTomatoExpressionInsectMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX Crk-SH2
Plasmid#46418DepositorInsertCrk (CRK Human)
UseTagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 1-124PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T Crkl SH3SH3
Plasmid#36403DepositorInsertCrkl tandem SH3 domains (CRKL Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDNA CrkII Y221F
Plasmid#50732Purposemammalian expression of CrkII Y221F mutantDepositorInsertCrkII Y221F (CRK Human)
UseTagsExpressionMammalianMutationY221FPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
CRKL gRNA (BRDN0001145388)
Plasmid#77508Purpose3rd generation lentiviral gRNA plasmid targeting human CRKLDepositorInsertCRKL (CRKL Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRKL gRNA (BRDN0001148341)
Plasmid#77509Purpose3rd generation lentiviral gRNA plasmid targeting human CRKLDepositorInsertCRKL (CRKL Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T Crkl SH2
Plasmid#36402DepositorInsertCrkl SH2 domain (CRKL Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 21, 2012AvailabilityAcademic Institutions and Nonprofits only