-
Plasmid#231566PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertdCas9-AsLOV2x3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-TASL-NEMO
Plasmid#221267PurposeExpression of TASL-NEMO IKK binding site in mammalian cells by retroviral transductionDepositorUseRetroviralTags3xFLAGExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-TASL(FL)
Plasmid#221263PurposeExpression of TASL in mammalian cells by retroviral transductionDepositorInsertTASL (Tasl Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-TASL-PLPLR
Plasmid#221266PurposeExpression of TASL-STING PLPLR in mammalian cells by retroviral transductionDepositorUseRetroviralTags3xFLAGExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3xHA-mASL
Plasmid#34577PurposeNOTE: This plasmid contains 1xHA-mASL, not 3xHA-mASL.DepositorInsertASL (Asl Mouse)
UseTags1xHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
CMV_dCas9-AsLOV2x3-NLSx3
Plasmid#231567PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertdCas9-AsLOV2x3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBbS5c-mCherry-AsLOV2*(543)
Plasmid#202712PurposeExpresses an IPTG inducible mCherry fused to a blue light responsive degradation tag (LOVdeg)DepositorInsertmCherry-AsLOV2*(543)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphasL-RLuc8
Plasmid#140981PurposeEncodes a G alpha subunit (GNAS1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphasL-RLuc8 (GNAS Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
1037 pGL3 FasL promoter
Plasmid#9028DepositorInsertFas ligand promoter (FASLG Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
CMV_Zdk3-VPR_P2A_dCas9-AsLOVx3-NLSx3
Plasmid#231568PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertAll-in-one LOOMINA construct
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-TASL(pLxIS)
Plasmid#221261PurposeExpression of TASL pLxIS (266-298) in mammalian cells by retroviral transductionDepositorInsertTASL pLxIS (266-298) (Tasl Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-TASL(Cterm)
Plasmid#221262PurposeExpression of TASL Cterm (102-298) in mammalian cells by retroviral transductionDepositorInsertTASL Cterm (102-298) (Tasl Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbA5k-AcrAB-AsLOV2*(543)
Plasmid#210863PurposeIPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg.DepositorInsertsacrA
acrB
UseSynthetic BiologyTagsAsLOV2*(543)ExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP-AsLOV2-NES27 (pDN158)
Plasmid#72659PurposeConstitutively nuclear variant of a very strong, photocaged NES (AsLOV2-NES 27) for blocking endogenous, CRM-1-dependent nuclear exportDepositorInsertH2B-GFP-AsLOV2-NES27
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FKBP-TASL(pLxIS-PLPLR)
Plasmid#221264PurposeExpression of TASL pLxIS-STING PLPLR in mammalian cells by retroviral transductionDepositorInsertTASL pLxIS-STING PLPLR (Tasl Mouse)
UseRetroviralTags3xFLAG-4xFKBPExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
1068 pGL3 FasL promoter (no SV40 prom)
Plasmid#9029DepositorInsertFas ligand promoter (FASLG Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationRemoved the SV40 promoter from pGL3PromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-FASLG-COMP5AP-AviTag-9xHis
Plasmid#157143PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertFASLG (FASLG Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-FASLG-Fc(DAPA)-AviTag-6xHis
Plasmid#156578PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertFASLG (FASLG Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Plasmid#92392Purposeproduces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTADepositorInsertTM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterHuman SynapsinAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
Plasmid#92390Purposeexpresses Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA component in mammalian cellsDepositorInsertTM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only