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Showing: 21 - 40 of 340 results

  3. pAAV-SynI-CreOn-FlpOff-MCS-P2A-EGFP

    Plasmid
    #176276
    Purpose
    Viral vector for co-expression of a CDS of interest and EGFP in cells expressing Cre AND NOT Flp driven by a Synapsin promoter. Contains an in-frame P2A sequence and an MCS for cloning of the CDS.
    Depositor
    Martin Riccomagno
    Article
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    MCS-P2A-EGFP
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin I
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    176276-plasmid-map-sequence-id-350687

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  4. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

    Plasmid
    #176279
    Purpose
    Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.
    Depositor
    Martin Riccomagno
    Article
    Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    Kir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
    Use
    AAV and Cre/Lox
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    GYG to AAA (aa144-146)
    Promoter
    human Synapsin I
    Available since
    Jan. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    176279-plasmid-map-sequence-id-350684

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  6. pOTTC1584 - pscAAV CMV-IE secreted EGFP-THPKTEL WPRE

    Plasmid
    #188539
    Purpose
    An AAV packaging vector that expresses secreted C-CDNF under control of the EGFP promoter.
    Depositor
    Brandon Harvey
    Insert
    secreted EGFP
    Use
    AAV
    Tags
    CDNF(1-28) and THPKTEL WPRE
    Expression
    Mutation
    Promoter
    CMV-IE
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188539-plasmid-map-sequence-id-370039

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  7. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60231
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and hSyn
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60231-plasmid-map-sequence-id-417804

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  8. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60230
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and hSyn
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    60230-plasmid-map-sequence-id-96396

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  9. pJEP325-pAAV-FullH1TO-SaCa9sgRNAi(CREB)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

    Plasmid
    #113702
    Purpose
    Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB. Followed by an EFS driven GFP-KASH in a separate reading frame.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 gRNA Cassete
    GFP
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    KASH
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    113702-plasmid-map-sequence-id-229517

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  11. pAAV-EF1a-N-CretrcintG

    Plasmid
    #69570
    Purpose
    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells
    Depositor
    Connie Cepko
    Article
    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do
    Has Service
    AAV1
    Insert
    kozak consensus - N-CretrcintG
    Use
    AAV and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69570-plasmid-map-sequence-id-127742

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  12. pAAV-EF1a-C-CreintG

    Plasmid
    #69571
    Purpose
    One half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cells
    Depositor
    Connie Cepko
    Article
    Tang et al Nat Neurosci. 2015 Sep;18(9):1334-41. do
    Has Service
    AAV1
    Insert
    kozak consensus - C-CreintG
    Use
    AAV and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Oct. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69571-plasmid-map-sequence-id-127747

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  16. pAAV.synP.DIO.EGFP.WPRE.hGH

    Plasmid
    #100043
    Purpose
    An AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).
    Depositor
    Ian Wickersham
    Has Service
    AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin-1
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100043-plasmid-map-sequence-id-193613

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  17. pAAV-SEO-CaMKII-EGFP

    Plasmid
    #177018
    Purpose
    For expression of EGFP in a single-floxed, excisable open reading frame (cre-off), under control of the CaMKIIa promoter
    Depositor
    Peter Jonas
    Insert
    EGFP
    Use
    AAV, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CaMKIIa
    Available since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177018-plasmid-map-sequence-id-353742

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Showing: 21 - 40 of 340 results