Showing: 21 - 40 of 1191 results
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Plasmid#80454Purposeenhanced SpCas9 mammalian expression vectorDepositorInsertehSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-HFSpCas9-H840A
Plasmid#80455Purposehigh fidelity SpCas9 mammalian expression vectorDepositorInsertHFSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pJWV102-PL-dCas9
Plasmid#85588PurposeIntegrate plasmid of Streptococcus pneumoniae, for chromosome integration of IPTG-inducible dCas9spDepositorInsertdCas9sp
UseCRISPRTagsExpressionBacterialMutationPromoterPLspAvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)-SPN+IFS
Plasmid#83372PurposeExpresses SPN and IFS complex in E. coli cellDepositorInsertsSPN
IFS
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-HeFSpCas9-H840A
Plasmid#80456Purposehigh fidelity enhanced SpCas9 mammalian expression vectorDepositorInsertHeFSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal
Plasmid#52694PurposeThis plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.DepositorInsertsCas9
Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
UseCRISPR; Toxoplasma gondiiTags3X FLAG and Nuclear localization signalExpressionMutationPromoterTgTUB1 and Toxoplasma U6AvailabilityAcademic Institutions and Nonprofits only -
pLZ12Km2-P23R:TA:ffluc
Plasmid#88900PurposeToxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expressionDepositorInserttoxin-antitoxin + firefly luciferase
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLZ12Km2-P23R:TA:fflucRT
Plasmid#88901PurposeToxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expressionDepositorInserttoxin-antitoxin + Red-shifted firefly luciferase
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLZ12Km2-P23R:TA:Xen5
Plasmid#88902PurposeToxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expressionDepositorInserttoxin-antitoxin + LuxABCDE
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pScCas9
Plasmid#117700PurposeMammalian expression vector encoding WT ScCas9DepositorInsertStreptococcus canis Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterEF-1αAvailabilityAcademic Institutions and Nonprofits only -
XClone_Cas9_KRAB_T2A_BFP
Plasmid#141208PurposeTunable and temporal expression control of dCas9DepositorInsertdCas9
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCRISPRx-Sth1Cas9-L5
Plasmid#140993PurposeSth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in MycobacteriaDepositorInsertsSth1Cas9
TetR
L5 attP
L5 Int
KanR
UseUnspecified; Integrative in mycobactreiaTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p3015b
Plasmid#209317PurposeExpresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.DepositorInsertGroup B Streptococcus-compatible single guide RNA
UseTagsExpressionBacterialMutationPromoterXyl/tet promoter that is constitutively activeAvailabilityAcademic Institutions and Nonprofits only -
pFG001
Plasmid#62816PurposeBacterial constitutive gRNA expression plasmidDepositorInsertgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPN25AvailabilityAcademic Institutions and Nonprofits only -
pFG018
Plasmid#62817PurposeBacterial inducible gRNA expression plasmidDepositorInsertgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailabilityAcademic Institutions and Nonprofits only -
pCMK
Plasmid#62818PurposeBacterial constitutive S. pyogenes Cas9 expression plasmidDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGEXndoS(E235Q)
Plasmid#44656DepositorInsertndoS (ndoS Streptococcus pyogenes)
UseTagsGstExpressionBacterialMutationChanged Glutamic acid 235 to GlutaminePromotertacAvailabilityAcademic Institutions and Nonprofits only -
pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)
Plasmid#171089PurposeCRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.DepositorInsertsDecoy sgRNA
dCas9
Chloramphenicol acetyltransferase
UseCRISPR; Toxoplasma gondii expressionTags3X FLAG and Nuclear Localization SignalExpressionMutationD10A, H840APromoterTUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…AvailabilityAcademic Institutions and Nonprofits only -
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XClone-Cas9-Cronus-T2A-BFP
Plasmid#141209PurposeTunable and temporal expression control of Cas9DepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 21 - 40 of 1191 results