-
Plasmid#39843DepositorInsertPlk1 (PLK1 Human)
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCer-C3-Plk1-T210D
Plasmid#68133PurposeExpression of Cerulean-tagged, constitutively active Plk1 in mammalian cellsDepositorInsertPolo-like kinase 1 (PLK1 Human)
UseTagsCeruleanExpressionMammalianMutationT210D constitutively active versionPromoterCMVAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCer-C3-Plk1-K82R
Plasmid#68134PurposeExpression of Cerulean-tagged, kinase-dead Plk1 in mammalian cellsDepositorInsertPolo-like kinase 1 (PLK1 Human)
UseTagsCeruleanExpressionMammalianMutationK82R kinase-dead versionPromoterCMVAvailable sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCS2-PLK1-mCherry
Plasmid#127154PurposeExpresses mCherry PLK1 in mammalian cells and zebrafish embryosDepositorInsertPLK1-mCherry (PLK1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMV IE94Available sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Plk1(829)
Plasmid#39845DepositorInsertPlk1 (PLK1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTXB3-Flag PLK1
Plasmid#68271Purposebacterial expression of Plk1DepositorInsertPLK1 (PLK1 Human)
UseTagsFlag and Mxe intein/chitin binding domainExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
PLK1 G5.1 gRNA
Plasmid#90834Purpose3rd generation lentiviral gRNA plasmid targeting human PLK1DepositorInsertPLK1 (Guide Designation G5.1)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PLK1 G6.1 gRNA
Plasmid#90835Purpose3rd generation lentiviral gRNA plasmid targeting human PLK1DepositorInsertPLK1 (Guide Designation G6.1)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-plk1-AKAP
Plasmid#132967PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damageDepositorUseTagsExpressionMammalianMutationC terminus of full-length Plk1 fused to the peric…PromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-p19-T7-PLK1
Plasmid#46312DepositorInsertsp19
PLK1 DNA-1
linker
PLK1 DNA-2
UsePro-sirnaTags6XHis and GSTExpressionBacterialMutationPromoterAvailable sinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
PLK1 gRNA (BRDN0001145759)
Plasmid#76457Purpose3rd generation lentiviral gRNA plasmid targeting human PLK1DepositorInsertPLK1 (PLK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PLK1 gRNA (BRDN0001144995)
Plasmid#76459Purpose3rd generation lentiviral gRNA plasmid targeting human PLK1DepositorInsertPLK1 (PLK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PLK1 gRNA (BRDN0001144743)
Plasmid#76458Purpose3rd generation lentiviral gRNA plasmid targeting human PLK1DepositorInsertPLK1 (PLK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PLK1 gRNA (BRDN0001147678)
Plasmid#76460Purpose3rd generation lentiviral gRNA plasmid targeting human PLK1DepositorInsertPLK1 (PLK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
membrane-HsPLK1-dead
Plasmid#185572Purposeto see protein dynamicsDepositorInsertPLK1-dead (PLK1 Human)
UseIn vitro transcription (mrna synthesis)Tagsmembrane targeting sequence from Neuromodulin (ML…ExpressionMutationK82R kinase-dead mutantPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Hec1 mCherry Plk1 T210D
Plasmid#45205DepositorInsertHec1-targeted mCherry Plk1 T210D
UseTagsmCherryExpressionMammalianMutationPlk1 T210D mutationPromoterT7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
Hec1 mCherry Plk1 K82R
Plasmid#45206DepositorInsertHec1-targeted mCherry Plk1 K82R
UseTagsExpressionMammalianMutationPlk1 K82R mutationPromoterT7Available sinceJuly 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(hPLK1)-PGKpuro2ABFP-W
Plasmid#163138PurposeLentiviral gRNA plasmid targeting human PLK1 gene, co-expression of BFP tagDepositorInsertPLK1 (PLK1 Human)
UseCRISPR and LentiviralTagsBFPExpressionMammalianMutationPromoterU6Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXB3-Flag PLK1 T210D
Plasmid#68272Purposebacterial expression of Plk1 T210D mutantDepositorInsertPLK1 (PLK1 Human)
UseTagsFlag and Mxe intein/chitin binding domainExpressionBacterialMutationT210DPromoterT7Available sinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRcCMV myc-Plk1 wt (Nigg RG6)
Plasmid#41160DepositorInsertPlk1 (PLK1 Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMarch 5, 2013AvailabilityAcademic Institutions and Nonprofits only