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Plasmid#214341PurposeProtein overexpression in bacteriaDepositorInsertIL1A (IL1A Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_CnCas12f1
Plasmid#204996PurposeCnCas12f1 heterologous expression plasmidDepositorInsertCnCas12f1
UseCRISPRTagsExpressionMutationPromoterT7Available sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.b201
Plasmid#131404PurposeE. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albuminDepositorInsertNanobody b201
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
Pet28a_Human_H3.3
Plasmid#42632DepositorInsertHuman H3.3 (H3-3A Human)
UseTagsUntaggedExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29_LuxSit_i
Plasmid#194299PurposeBacterial expression of the de novo luciferase, LuxSit-iDepositorInsertLuxSit-i
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
HotPETase_pET21b
Plasmid#215407PurposeExpression construct for HotPETase gene, codon optimized for expression in E. coliDepositorInsertHotPETase
UseTagsExpressionBacterialMutationS121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…PromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28c-Ku
Plasmid#161936PurposeExpresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purificationDepositorInsertku (mku Mycobacterium tuberculosis)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-37
Plasmid#214346PurposeProtein overexpression in bacteriaDepositorInsertIL37 (IL37 Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET283xFlagPP7CPHis
Plasmid#28174DepositorInsertPP7 coat protein optimized
UseTags6xhistidine and triple FLAG peptideExpressionBacterialMutationC68A,C73APromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAR18_StrepI_mFASm_H8_pET22b
Plasmid#122847PurposeExpresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInsertmouse FASN/ mouse fatty acid synthase (Fasn Mouse)
UseTagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET29b_mNeonGreen
Plasmid#209425PurposeExpress mNeonGreen in E. coliDepositorInsertmNeonGreen
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a_MBP_RBFOX2
Plasmid#224292PurposeMBP-RBFOX2 protein purification from bacteriaDepositorInsertRNA binding fox-1 homolog 2 (RBFOX2 Human)
UseTags6xHis and MBPExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-33
Plasmid#214342PurposeProtein overexpression in bacteriaDepositorInsertIL33 (IL33 Human)
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
FsC_pET29b
Plasmid#215405PurposeExpression construct for FsC gene, codon optimized for expression in E. coliDepositorInsertFsC
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28b_Q69L
Plasmid#186272PurposeExpression vector of His-RanQ69L in E.coliDepositorInsertGTP-binding nuclear protein Ran (Ran Mouse)
UseTagsHis-tagged at N terminalExpressionBacterialMutationChanged Q69 into LPromoterT7 promoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_3
Plasmid#78200PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,I76G,A197C)
UseTagsExpressionBacterialMutationA17C,I76G,A197C (please see depositor comment bel…PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
ELK3_pet28a
Plasmid#131651PurposeBacterial expression of ETS gene ELK3DepositorInsertELK3 (ELK3 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAR17_StrepI_hFASh_H8_pET22b
Plasmid#122846PurposeExpresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInserthuman FASN/ human fatty acid synthase (FASN Human)
UseTagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28Teb1
Plasmid#28173DepositorInsertTeb1
UseTags6xhistidineExpressionBacterialMutationPromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
UseTagsExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only