Addgene

We narrowed to 98 results for: OFP

Showing: 21 - 40 of 98 results

Results per page:

pAS1-rab-3P::his-24::CyOFP

Plasmid

#124347

Purpose

Nuclear Localized Pan-Neuronal CyOFP

Depositor

Andrew Leifer

Insert

rab-3, CyOFP (rab-3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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L2125-FRB-GPA-NLuc-CyOFP1

Plasmid

#222885

Purpose

Positive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc-10GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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L2127-FRB-GPA-114-20-CyOFP1

Plasmid

#222886

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114-20GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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L2128-FRB-GPA-114-10-CyOFP1

Plasmid

#222887

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114-10GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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L2129-FRB-GPA-114-5-CyOFP1

Plasmid

#222888

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc114-5GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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L2130-FKBP-GPA-114-20-CyOFP1

Plasmid

#222889

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc114-20GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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L2131-FKBP-GPA-114-10-CyOFP1

Plasmid

#222890

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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L2132-FKBP-GPA-114-5-CyOFP1

Plasmid

#222891

Purpose

Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FKBP-GPA-20GS-NanoLuc114-5GS-CyOFP1

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

Plasmid

#75154

Purpose

Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3-P2A-mKOFP2-CAAX

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Availability

Academic Institutions and Nonprofits only

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p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

Plasmid

#75177

Purpose

Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3-P2A-mKOFP2-CAAX

Use

Three prime entry vector for multisite gateway th…

Tags

Expression

Mutation

Promoter

n/a

Availability

Academic Institutions and Nonprofits only

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iCas

Plasmid

#84232

Purpose

Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

Depositor

Meng How Tan

Article

Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

Insert

Cas9

Use

Tags

2A-OFP co-expression and ERT2-ERT2

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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iCas

Plasmid

#84232

Purpose

Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

Depositor

Meng How Tan

Article

Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

Insert

Cas9

Use

Tags

2A-OFP co-expression and ERT2-ERT2

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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LV-Let7d-5p

Plasmid

#117055

Purpose

Overexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expression

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

Let-7d-5p - OFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV promoter

Availability

Academic Institutions and Nonprofits only

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LV-miR-142

Plasmid

#117056

Purpose

Overexpression of miR-142-3p using miR-451 backbone to enable DICER-independent expression along with orange fluorescent protein (OFP) to track miRNA expression

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

miR-142-3p - OFP (Mir142 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV promoter

Availability

Academic Institutions and Nonprofits only

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LV-Let7d-5p

Plasmid

#117055

Purpose

Overexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expression

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

Let-7d-5p - OFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
LV-miR-142

Plasmid

#117056

Purpose

Overexpression of miR-142-3p using miR-451 backbone to enable DICER-independent expression along with orange fluorescent protein (OFP) to track miRNA expression

Depositor

Michele De Palma

Article

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

miR-142-3p - OFP (Mir142 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV promoter

Availability

Academic Institutions and Nonprofits only

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pPKm-102

Plasmid

#90493

Purpose

pcDNA3 - CMVmin - mOrange, plasmid expressing orange fluorescent protein (OFP)

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mOrange

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pPKm-243

Plasmid

#90506

Purpose

mOrange and mitochondrial-tagged sfGFP (pSIN-OFP-mitosfGFP)

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mOrange

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTiGc

Plasmid

#78314

Purpose

Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).

Depositor

Samantha Sampson

Article

Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/

Inserts

TurboFP635
GFP

Use

Synthetic Biology; Mycobacterial replicating vect…

Tags

Expression

Bacterial

Mutation

Promoter

hsp60 and hsp60(ribo)

Availability

Academic Institutions and Nonprofits only
pUT1863-HQ858798

Plasmid

#27880

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

OFP transcription factor from DV526477

Use

Entry vector

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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