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Plasmid#70428PurposeGateway ORF clone of human NFKB1 [NM_003998.3] without stop codon (for C-terminal fusions)DepositorInsertNFKB1 (NFKB1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-NFKB1-PGK-EGFP
Plasmid#204018PurposeHomologous donor template for NFKB1 knockout expressing EGFPDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-NFKB1-PGK-tdTomato
Plasmid#204019PurposeHomologous donor template for NFKB1 knockout expressing tdTomatoDepositorInserttdTomato
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-NFKB2-PGK-EGFP
Plasmid#204020PurposeHomologous donor template for NFKB2 knockout expressing EGFPDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-NFKB2-PGK-tdTomato
Plasmid#204021PurposeHomologous donor template for NFKB2 knockout expressing tdTomatoDepositorInserttdTomato
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSI-Check2-hRluc-NFkB-firefly
Plasmid#106979PurposeLuciferase reporter with internal control for NFkB signal.DepositorInsertNFKB recognition sites (NFKB1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE NFkB-TA-LUC-UBC-GFP-W
Plasmid#49343PurposepHAGE NFkB-TA-LUC-UBC-GFP-WDepositorInsertsUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE NFKB-TA-LUC-UBC-dTomato-W
Plasmid#49335PurposepHAGE NFKB-TA-LUC-UBC-dTomato-WDepositorInsertsNFkB consensus binding sequence
TA
Luciferase
UBC
dTomato
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-Tornado-F30-Broccoli-aptNFkB#5
Plasmid#124362PurposeOverexpression of bifunctional circular RNA (Broccoli fluorescence and NFkB binding)DepositorInsertTornado-F30-Broccoli-aptNFkB#5
UseTagsExpressionMammalianMutationPromoterU6+27Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
LB1461 (Bcl-2 promoter with MHS1234, NFkB mut)
Plasmid#15385DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterial and MammalianMutationBcl-2 full promoter with IgH full enhancer MHS123…PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9
Plasmid#223163Purpose2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA ScaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterAvailable sinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2
Plasmid#223164Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffoldDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2047-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)
Plasmid#223167Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOEDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2045-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(CMV-gRNA1)
Plasmid#223165Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting CMVDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK2046-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)
Plasmid#223166Purpose2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOEDepositorInsertKRAB-MECP2 (MECP2 Human)
UseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)PromoterEF1aAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mutLuc
Plasmid#106458Purposeluciferase reporter, CMV promoter (3 NFkB sites mutated)DepositorInsertluciferase reporter, CMV promoter (3 NFkB sites mutated)
UseLuciferaseTagsExpressionMammalianMutation3 NFkB sites mutatedPromoterAvailable sinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-CREM2 luc
Plasmid#106460Purposeluciferase reporter, CMV promoter (CRE site adjacent to NFkB site mutated)DepositorInsertluciferase reporter, CMV promoter (CRE site adjacent to NFkB site mutated)
UseLuciferaseTagsExpressionMammalianMutationCRE site adjacent to NFkB site mutatedPromoterAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
p1242 3x-KB-L
Plasmid#26699DepositorInsert3x MHC I KB element - luciferase (NFKB1 Photinus pyralis, Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pGL3Luc-5XNF-kappaB
Plasmid#185695PurposeReporter plasmid with a double-strand oligonucleotides that contain five repeats of NF-kappaB binding consensus sequence (5'-GGGGAATTTCC-3').DepositorInsertNF-kappaB (NFKB1 Human)
UseLuciferaseTagsExpressionMutationPromoterNF-kappaB binding siteAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only