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Plasmid#113517PurposeExpresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nucleaseDepositorInsertLbCas12a
UseTagsMBPExpressionBacterialMutationD832APromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a H759A
Plasmid#113518PurposeExpresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processingDepositorInsertLbCas12a
UseTagsMBPExpressionBacterialMutationH759APromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-DR-GC-swap-cr in pBCSK+
Plasmid#126643PurposeCloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cellsDepositorInsertLbCas12a Full Length Direct Repeat GC Swap crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 LbCas12a (GB1439)
Plasmid#160573PurposeHuman codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).DepositorInsertLbCas12a
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
nLbCas12a
Plasmid#213745PurposeFor circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cellsDepositorInsertnLbCas12a
UseCRISPRTagsBPNLSExpressionMammalianMutationD156R, R1138APromoterCMVAvailable sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCJH027_SC101_pLAC_LbCas12a_Kan
Plasmid#183074PurposeWild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.DepositorInsertLbCas12a
UseTagsExpressionBacterialMutationWild-typePromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha1 P35S:LbCas12a:T35S (GB1441)
Plasmid#160611PurposeTranscriptional unit for the expression of the LbCas12a in plant systems driven by the CaMV35s promoter.DepositorInsertP35s:LbCas12a:T35s
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterP35SAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtU6-26:LbCas12a_DR (GB1443)
Plasmid#160575PurposeLbCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.DepositorInsertAtU6-26:LbCas12a_DR
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterU6-26Available sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12aD156R
Plasmid#164659PurposeBacterial expression of mutated LbCas12aD156R using CL7/Im7DepositorInsertLbCas12a-D156R
UseTagsThioredoxin, CL7, SUMOExpressionBacterialMutationchanged Aspartic Acid 156 to ArgininePromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
rnLbCas12a-PE2
Plasmid#213746PurposeFor circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cellsDepositorInsertrnLbCas12a-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianMutationD156R, H759A, R1138A in LbCas12aPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNOC_hlbCas12a-Nlux
Plasmid#176240PurposepNOC episomal plasmid harboring the humanized lbCas12a gene sequence tagged with Nlux under the control of Ribi promoterDepositorInserthumanized lbCas12a
UseCRISPR, Luciferase, and Synthetic Biology ; Expre…TagsluciferaseExpressionMutationPromoterRibosomal subunitAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-DR-cr in PBCSK-
Plasmid#126641PurposeCloning vector LbCas12a DR-crRNA for expression in mammalian cellsDepositorInsertLbCas12a Full Length Direct Repeat crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Topo_SpCas9.tracr_LbCas12a.DR
Plasmid#155049PurposeTOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vectorDepositorInsertSpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)
Plasmid#160629PurposeTU for the expression of LbCas12aDepositorInsertAtUBQ10-:LbCpf1(Cas12a):Tnos
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterUBQ10Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-D156R (ttLbCas12a)
Plasmid#195366PurposeA temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12aDepositorInsertLbCas12a-D156R (ttLbCas12a)
UseCRISPR; Gateway compatible lbcas12a-d156r (ttlbca…TagsExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGT8163 (AATG_ttLbCas12a-i_TTCG)
Plasmid#192453Purposeintronized ttLbCas12a for in planta expressionDepositorInserttemperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
UseCRISPR and Synthetic Biology; Moclo compatible le…TagsExpressionMutationtemperature-tolerant mutation (D156R)PromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-2C-NLS in pCSDest
Plasmid#126638PurposeExpresses LbCas12a-2C-NLS in mammalian cellDepositorInsertLbCas12a-2C-NLS
UseCRISPRTags3xHuman influenza hemagglutinin (HA)-TagExpressionMammalianMutationPromoterCMV IE94 promoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a D832A
Plasmid#113517PurposeExpresses His-MBP fused to LbCas12a with D832A mutation to inactivate RuvC nucleaseDepositorInsertLbCas12a
UseTagsMBPExpressionBacterialMutationD832APromoterT7Available sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGT8162 (AATG_dttLbCas12a-i_TTCG)
Plasmid#192350PurposeL0 MoClo-compatible GoldenGate-module of dttLbCas12a-i for in planta expressionDepositorInsertAATG_dttLbCas12a-i_TTCG
UseCRISPRTagsExpressionMutationD156R; E925QPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBP-LbCas12a H759A
Plasmid#113518PurposeExpresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processingDepositorInsertLbCas12a
UseTagsMBPExpressionBacterialMutationH759APromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only