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Plasmid#196911Purposevisualization of clathrin-coated vesicle in living mammalian cellsDepositorInsertClathrin-Coated light chain
UseTagsHcRedExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPM1cdN243-C1
Plasmid#131838PurposeExpresses the last 50 amino acids of HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsHcRedExpressionMammalianMutationThis is a deletion contruct of the cytoplasmic m…PromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPM1cOligodN243-C1
Plasmid#131847PurposeExpresses HcRed-NPM1c (cytoplasmic mutant, human) with the oligo dimerization domain attached to the last 50 aminoacids in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsHcRedExpressionMammalianMutationThis is a deletion contruct of the cytoplasmic m…PromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPM1cNLSdN243-C1
Plasmid#131848PurposeExpresses HcRed-NPM1c (cytoplasmic mutant, human) with the NLS domain (aa. 121-195) attached to the last 50 aminoacids in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsHcRedExpressionMammalianMutationThis is a deletion contruct of the cytoplasmic m…PromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPMMLF1nes-C1
Plasmid#131849PurposeExpresses the N-terminal nuclear export signal of HcRed-NPMMLF1 in mammalian cells through transient transfectionDepositorInsertNPM1 (NPM1 Human)
UseTagsHcRedExpressionMammalianMutationThis is a control deletion contruct of the cytop…PromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEX-HcRed-hLC3WT
Plasmid#24991DepositorInserta wild type human MAP1-LC3B fused with HcRed (MAP1LC3B Human)
UseTagsHcRedExpressionMammalianMutationPromoterAvailable sinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBabe HcRed Ras
Plasmid#10678DepositorInsertHras V12 (HRAS Human)
UseRetroviralTagsExpressionMammalianMutationG12V, constitutively activePromoterAvailable sinceNov. 14, 2005AvailabilityAcademic Institutions and Nonprofits only -
pEX- HcRed -hLC3ΔG
Plasmid#24992DepositorInserta mutant human MAP1-LC3B fused with HcRed (MAP1LC3B Human)
UseTagsHcRedExpressionMammalianMutationdeleted Gly at 121PromoterAvailable sinceJune 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-miR-125b-1
Plasmid#58989PurposeMSCV based expression vector - co-expresses HcRed and human miR-125b-1DepositorInsertsUseRetroviralTagsExpressionMammalianMutationPromoterMSCVAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
Mucolipin1 D471-472K-pHcRed C1
Plasmid#62961Purposefull length human Mucolipin-1 D471/472K cloned into pHcRed1 C1DepositorInsertMucolipin-1 (MCOLN1 Human)
UseTagsHcRedExpressionMammalianMutationD471/472K (and two silent mutations T77 ACA inst…PromoterCMVAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-hcRed-V5-miniTurbo_IDG-K
Plasmid#135237PurposeGateway destination clone of hcRed (as control) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInserthcRed-V5-miniTurbo
UseLentiviral; Gateway destinationTagsV5-miniTurboExpressionMammalianMutationPromoterUbiquitinAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-hcRed-V5-TurboID_IDG-K
Plasmid#135238PurposeGateway destination clone of hcRed (as control) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInserthcRed-V5-TurboID
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-hcRed-V5_IDG-K
Plasmid#135239PurposeGateway destination clone of hcRed (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInserthcRed-V5
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-hcRed_IDG-K
Plasmid#135274PurposeGateway destination clone of hcRed (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-hcRed
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
H2B-RFP in pENTR1A (w507-1)
Plasmid#22525DepositorInsertHuman Histone H2B -Heteractis crispa red fluorescent protein (H2BC21 Human)
UseEntry vectorTagsHcRedExpressionMutationPromoterAvailable sinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
PJEBAN4
Plasmid#203683PurposeExpresses red fluorescent marker HcRed in Listeria monocytogenesDepositorInsertHcRed
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRFP
Plasmid#26924DepositorInsertLacI, HcRed
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
U6::sgBFP-U6::sgKrasG12D-pX333-red
Plasmid#149448PurposepX333 backbone expressing 2 sgRNA targeting BFP and KRAS G12D to edit mouse KRAS G12D allele.DepositorInsertsgRNA BFP and mouse KRAS G12D
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBMN-dGBP1-TagBFP
Plasmid#80087PurposeMMLV retroviral vector for expression of dGBP1-TagBFP with t-hcRed as an infection/transfection markerDepositorInsertdGBP1-TagBFP
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBMN HAYFP-LOV24
Plasmid#49570Purposeregulate protein stability with lightDepositorInsertYFP-LOV24
UseRetroviralTagsHA and YFPExpressionMutationdelete the last 3 aa from AsLOV2, with I532A muta…PromoterLTRAvailable sinceFeb. 19, 2014AvailabilityAcademic Institutions and Nonprofits only