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Plasmid#17518DepositorInsertB. subtilis DEAD-box helicase YxiN
UseTagsintein-chitin binding domainExpressionBacterialMutationE152Q mutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
DGP104- pSP64 Poly(A)700
Plasmid#70098Purposean RNA spike-in with similar GC content to S. cerevisiae mRNAs, for in vivo measurement of RNA kineticsDepositorInsert700bp insert from B. subtilis
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6Available sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWH-DI
Plasmid#47385PurposeExpresses dxs and idi under PxylA promoter (inducible by xylose) in bacteriaDepositorInsertsdxs
idi
UseTagsExpressionBacterialMutationPromoterPxylAAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
Sfp pet29b C-terminal His Tag
Plasmid#75015PurposeExpresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACPDepositorInsertSfp
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB3
Plasmid#82027PurposeHybrid McpC[1-350]-Tar[267-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-350]-Tar[267-553]
UseTagsExpressionMutationPromoterAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB2
Plasmid#82026PurposeHybrid McpC[1-286]-Tar[200-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-286]-Tar[200-553]
UseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB6
Plasmid#82028PurposeHybrid McpC[1-350]-YSYEH-Tar[272-553]DepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: McpC[1-350]-YSYEH-Tar[272-553]
UseTagsExpressionMutationPromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a207-479.wt
Plasmid#17516DepositorInsertB. subtilis DEAD-box helicase YxiN second domain
UseTagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 1-206PromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a1-368.E152Q
Plasmid#17517DepositorInsertB. subtilis DEAD-box helicase YxiN first two domains
UseTagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 369-479; E152Q mutat…PromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a207-368.wt
Plasmid#13531DepositorInsertB. subtilis DEAD-box helicase YxiN second domain
UseTagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 1-206 and 369-479PromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSB1
Plasmid#81227PurposeHybrid Tar[1-43]-McpC[49-270]-Tar[184-553] expression plasmidDepositorInsertEncodes the hybrid receptor of E. coli chemoreceptor Tar and B. subtilis chemoreceptor McpC: Tar[1-43]-McpC[49-270]-Tar[184-553]
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
Pveg
Plasmid#55173PurposeVery strong constitutive promoterDepositorInsertveg promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromotervegAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
PxylA
Plasmid#55178PurposeXylose-inducible promoterDepositorInsertxylA promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterxylAAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
PlepA
Plasmid#55175PurposeStrong constitutive promoterDepositorInsertlepA promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHT-6xR.ads-PxylA-DI
Plasmid#47386PurposeExpresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteriaDepositorInsertsads
dxs
idi
UseTags6xR and HisExpressionBacterialMutationPromoterPgrac and PxylAAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
PliaI
Plasmid#55177PurposeBacitracin-inducible promoterDepositorInsertliaI promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterliaIAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
PliaG
Plasmid#55174PurposeConstitutive promoterDepositorInsertLiaG promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Trx-His6-TEV_SacB
Plasmid#164588PurposeBacterial expression plasmid with sacB gene. Suitable for LIC cloning of a gene insert replacing the sacB.DepositorInsertTEV-SacB
UseTagsTrx-His6 followed by TEV siteExpressionBacterialMutationPromoterT7Available sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBP-Pspac
Plasmid#112778PurposePromoter repressed by LacI (not natively produced in B. subtilis 168)DepositorArticleInsertPspac promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSWEET-bgaB
Plasmid#210272PurposeXylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.DepositorInsertbgaB
UseTagsExpressionBacterialMutationPromoterPxylAAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only