1. p426 72Q GPD

   Plasmid

   #1183

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 72Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

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1. p2R3z-BASI-ccdB-BSAI

   Plasmid

   #118389

   Purpose

   Entry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   ccdB
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GFP-Epac1 SAAX

   Plasmid

   #118313

   Purpose

   Expression of human Epac1

   Depositor

   Philip Stork

   Article

   Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

   Insert

   Epac1 SAAX (RAPGEF3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 2Flag-pDEST-N

   Plasmid

   #118371

   Purpose

   Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Type

   Empty backbone

   Use

   Destination

   Tags

   2Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pENTR-actin

   Plasmid

   #118378

   Purpose

   Gateway compatible donor vector with WT actin.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. hSUMO1 GFP

   Plasmid

   #118360

   Purpose

   This plasmid expresses the full length human SUMO1.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hSUMO1 (SUMO1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3-CrmA

   Plasmid

   #11832

   Depositor

   Guy Salvesen

   Article

   Muzio et al J Biol Chem. 1997 Jan 31. 272(5):2952-6.

   Insert

   CrmA (CrmA Cowpox virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GFP-PKI nls

   Plasmid

   #118301

   Purpose

   Expression of PKIa

   Depositor

   Philip Stork

   Article

   Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

   Insert

   PKI nls (aa 2-36)
   

   Use

   Tags

   Flag and GFP

   Expression

   Mammalian

   Mutation

   inserting 2xnls

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-PKI WT

   Plasmid

   #118300

   Purpose

   Expression of PKIa

   Depositor

   Philip Stork

   Article

   Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

   Insert

   PKI wt
   

   Use

   Tags

   Flag and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR-NLS-actin

   Plasmid

   #118380

   Purpose

   Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Tags

   Expression

   Mutation

   NLS

   Promoter

   Available since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p221z-CAS9p-t35s

    Plasmid

    #118385

    Purpose

    Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    CAS9p
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSUPER-scramble

    Plasmid

    #118349

    Purpose

    This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    scrambled shRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p2R3z-AtU3b-tRNA-ccdB-gRNA

    Plasmid

    #118390

    Purpose

    Entry clone expressing gRNA. Target site can be introduced between tRNA and gRNA backbone. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    AtU3b promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mCherry-Rap1b

    Plasmid

    #118319

    Purpose

    Expression of bovine Rap1b

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Insert

    Rap1b (RAP1B Bovine)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. hHSF1 S303/7A

    Plasmid

    #118365

    Purpose

    This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S303A and S307A

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. GFP-RalGDS

    Plasmid

    #118315

    Purpose

    Expression of human RalGDS

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Insert

    RalGDS (RALGDS Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    fragment of human RalGDS 203aa

    Promoter

    CMV

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSUPER-hHSF1 shRNA

    Plasmid

    #118345

    Purpose

    This plasmid expresses shRNA against hHSF1.

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF1 shRNA (HSF1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Flag-Craf R89L

    Plasmid

    #118333

    Purpose

    Expression of human Craf

    Depositor

    Philip Stork

    Article

    Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

    Insert

    Craf R89L (RAF1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R89L

    Promoter

    CMV

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hGATA-1 K137R

    Plasmid

    #118353

    Purpose

    This plasmid expresses human GATA-1 K137R mutant. This construct cannot undergo sumoylation on K137.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hGATA-1 (GATA1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    K137R

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p221z-dCAS9p-t35s

    Plasmid

    #118387

    Purpose

    Entry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    dCAS9p
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-Rap2b

    Plasmid

    #118321

    Purpose

    Expression of human Rap2b

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Insert

    Rap2b (RAP2B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge