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PTGS1-bio-His
Plasmid#53414PurposeExpresses full-length Prostaglandin G/H synthase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPTGS1 (PTGS1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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F11-R pEBio
Plasmid#61486PurposeExpresses full-length F11R (JAM1) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag and biotinylation sequenceDepositorInsertF11R (F11R Human)
UseTagsenzymatic biotinylation sequence and rat CD4 d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2-Cx43-siResist
Plasmid#49852PurposeEncodes human connexin 43 with wobble mutations conferring resistance against an siRNADepositorInsertConnexin 43 (GJA1 Human)
UseTagsExpressionMammalianMutationContains several wobble mutations to confer resis…PromoterCMVAvailable sinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE-Flag-Cdk9-D167N-IRES-eGFP
Plasmid#28098DepositorInsertCdk9 (CDK9 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationContains D167N mutation in Cdk9PromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
MERS-coV-NSP3-c001
Plasmid#173085PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep (orf1ab Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV))
UseTagsmCeruleanExpressionBacterialMutationD1109-D1275, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO-rc[Chronos-eGFP]-WPRE
Plasmid#203847PurposeFlp-dependent expression of Chronos fused to EGFPDepositorInsertChronos
UseAAVTagsEGFPExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
F2-bio-His
Plasmid#52179PurposeExpresses full-length Prothrombin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertF2 (F2 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
PTPRJ-bio-His
Plasmid#51816PurposeExpresses full-length Receptor-type tyrosine-protein phosphatase eta precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPTPRJ (PTPRJ Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
PPIB-bio-His
Plasmid#53413PurposeExpresses full-length Peptidyl-prolyl cis-trans isomerase B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPPIB (PPIB Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 T7 DLC1
Plasmid#24266DepositorUseTagsT7ExpressionMammalianMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBrain-GFP-FKBP-TACC3-shTACC3
Plasmid#59354PurposeKnocks down endogenous TACC3 and expresses RNAi-resistant human TACC3 tagged with GFP & FKBP in mammalian cells.DepositorInsertTransforming Acidic Coiled Coil protein 3 (TACC3 Human)
UseRNAiTagsEGFP and FKBPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBabe EGFR ASVinsertion
Plasmid#32066DepositorInsertEGFR (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationInsert duplicate A767-V769PromoterAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIS1-Vim/Actb5UTR-renilla
Plasmid#38238DepositorUseLuciferaseTagsExpressionMammalianMutationActb promoter (1000 nt upstream) and 5' UTR …PromoterEndogenous VimAvailable sinceNov. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pJZC48
Plasmid#62336PurposesgRNA + 1x COM with COM-VP64 effector for mammalian cellsDepositorInsertssgRNA + 1x COM binding module
COM-VP64
UseLentiviralTagsVP64ExpressionMammalianMutationTargets Tet3G, sequence: GTACGTTCTCTATCACTGATAPromoterCMV and U6Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9
Plasmid#84242PurposeExpresses GA-inducible KRAB-Sp dCas9DepositorInsertsGAI-tagBFP-Sp dCas9
GID1-KRAB
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCK341
Plasmid#192640PurposeExpresses dSpRY and MCP-SoxS(R93A/S101A) on p15A-CmRDepositorInsertsSp.pCas9-dSpRY
BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPR and Synthetic BiologyTagsExpressionMutationSoxS has R93A and S101A mutations and dSpRY has s…PromoterBBa_J23107 and Sp.pCas9Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
