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Plasmid#35700PurposeTo fuse your protein of interest to the C-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID); myc tagDepositorHas ServiceCloning Grade DNAInsertBirA
UseTagsMycExpressionMammalianMutationR118G (highly promiscuous form)PromotercmvAvailable sinceApril 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-D1ER
Plasmid#36325DepositorInsertD1ER second generation cameleon (calcium sensor) targeted to ER
UseTagsKDELExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA Sam68 WT
Plasmid#17690DepositorInsertSam68 (Khdrbs1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-6XHis-human SUMO 2
Plasmid#133771Purposeexpress 6Xhis- SUMO 2 proteinDepositorInsertSUMO 2 (SUMO2 Human)
UseTags6XHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBudORF2-CH
Plasmid#51289Purposeexpresses the codon optimized ORF2 of L1RP with his tagDepositorInsertL1 ORF2 (L1RE1 Human)
UseTagsHis and MycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL-pRc/CMV
Plasmid#19999DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseTagsHA-tagExpressionMammalianMutationwild typePromoterAvailable sinceApril 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBAD-mhYFP
Plasmid#186532PurposeMonomeric hyperfolder YFP fluorescent protein: bacterial expression plasmidDepositorInsertMonomeric hyperfolder YFP
UseTags6xHisExpressionBacterialMutationhfYFP-S147P/L195M/V206KPromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Lifeact EGFP
Plasmid#187686PurposeIn vivo visualization of actinDepositorInsertLifeact
UseLentiviralTagsEGFPExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRVL-4
Plasmid#104582PurposeContains human kappa CL domain, for cloning of antibody VL domains using SapI restriction enzyme.DepositorInsertHuman CL kappa
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
gTEMP_pcDNA3
Plasmid#89583PurposeGenetically encoded ratiometric fluorescent thermometerDepositorInsertgTEMP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-6XHis-human SUMO 1
Plasmid#133770Purposeexpress 6Xhis- SUMO 1 proteinDepositorInsertSUMO 1 (SUMO1 Human)
UseTags6XHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL4A-FLAG
Plasmid#15821DepositorInsertCullin4A (CUL4A Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N440PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M2
Plasmid#135583PurposeHigh eukaryotic protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL5-FLAG
Plasmid#15823DepositorInsertCullin5 (CUL5 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N441PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-f:PGC1
Plasmid#1026DepositorInsertPPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-6Xhis-human SUMO 3
Plasmid#133772Purposeexpress 6Xhis- SUMO 3 proteinDepositorInsertSUMO 3 (SUMO3 Human)
UseTags6XHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL1-FLAG
Plasmid#15818DepositorInsertCullin1 (CUL1 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N452PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBad-CA
Plasmid#105665Purposeexpresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tagDepositorArticleInserthuman carbonic anhydrase II thermostable variant (CA2 Human)
UseTags6x his tagExpressionBacterialMutationPromoterpBad/arabinoseAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DN-hCUL2-FLAG
Plasmid#15819DepositorInsertCullin2 (CUL2 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N427PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TFEBS211A-MYC (#805)
Plasmid#99957Purposemammalian expressionDepositorInsertTFEB (TFEB Human)
UseTags6xHIs and MycExpressionMammalianMutationS211APromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only