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Plasmid#25103DepositorInsertpro-SUMO3 (SUMO3 Human)
UseTagsHisExpressionBacterialMutationchanged Serine 2 to AlaninePromoterAvailable sinceJune 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R49A)
Plasmid#112726PurposeArg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R49A)
UseTags6His-TEVExpressionBacterialMutationR49APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-2HMG-mCherry
Plasmid#112739PurposeExpress 2HMG-mCherry in E. coliDepositorInsert2HMG-mCherry
UseTags6xHisExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-TGFB3m
Plasmid#120289PurposeProduce human recombinant mTGFB3 in DE3 cellsDepositorInsertmonomeric Transforming Growth Factor-β3
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mH6-Cas12i2
Plasmid#120883PurposeExpresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.DepositorInsertCas12i2
UseCRISPRTagsmH6ExpressionBacterialMutationWTPromoterlacAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS_0143-pET30-Fusicatenibacter_saccharivorans
Plasmid#116952PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3991 pET ubcH9
Plasmid#8651DepositorInsertUbcH9 (UBE2I Human)
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
BioBrick pET-24a(+)
Plasmid#117802PurposeBioBrick pET vector for protein overexpression in E. coliDepositorTypeEmpty backboneUseEscherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-TGFB1m
Plasmid#120286PurposeProduce human recombinant mTGFB1 in DE3 cellsDepositorInsertmonomeric Transforming Growth Factor-β1
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromotorAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmBelle_B
Plasmid#145963PurposeBacterial Expression of DmBelleDepositorInsertDmBelle (bel Fly)
UseTagsExpressionBacterialMutationdel 155-157, T530A and I685VPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla 3A
Plasmid#84872PurposeBacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
Fla
UseTagsHisExpressionBacterialMutation3 C-terminal leucines have been replaced by alani…PromoterT7Available sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21b-LOV-ipaA
Plasmid#40236DepositorInsertLOV-ipaA
UseTags6xHisExpressionBacterialMutationJα helix region hybridized (AsLOV2 aa 537-546/ipa…PromoterT7Available sinceSept. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del4 pET28C
Plasmid#122118PurposeExpression Brg1 amino acids 1446-1647 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Brg1 Del3 pET28a
Plasmid#122117PurposeExpression Brg1 amino acids 836-1446 in bacteriaDepositorInsertBrg1 (SMARCA4 Human)
UseTagsHis-T7ExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET32a-Trx-PP-hNF2
Plasmid#107148Purposebacterial expressionDepositorInsertneurofibromin 2 (NF2 Human)
UseTags(His)6-ThioredoxinExpressionBacterialMutationPromoterT7Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
883 pET15b PTEN
Plasmid#10936DepositorInsertPTEN (PTEN Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-24a-CXCR4
Plasmid#133431PurposeHuman CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.DepositorInsertCXCR4 (CXCR4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
5787 pET28aSX104B/ pES42
Plasmid#1229DepositorInsertHsp104 (HSP104 Budding Yeast)
UseTagsHisExpressionBacterialMutationnonePromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
CBP-HAT-pET
Plasmid#21091DepositorInsertCBP (Crebbp Mouse)
UseTagsHis and T7 tagExpressionBacterialMutationHAT domain aa 1171-1621PromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET22b-BirA-CPDSalI
Plasmid#38258DepositorInsertbirA (birA E. coli)
UseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationcloned from pGEX4T1-BirAPromoterT7Available sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only