Showing: 3941 - 3960 of 3967 results
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Plasmid#168127PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein G13. Composed of the subunits G alpha 13 (GNA13) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at F125/D126 within GNA13PromoterAvailabilityAcademic Institutions and Nonprofits only
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Go1-CASE
Plasmid#168123PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).DepositorUseLuciferaseTagscpVenus on GNG9ExpressionMammalianMutationNLuc is inserted at L91/G92 within GNAO1PromoterAvailabilityAcademic Institutions and Nonprofits only -
Gs(short)-CASE
Plasmid#168124PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).DepositorUseLuciferaseTagscpVenus on GNG1ExpressionMammalianMutationNLuc is inserted at N136/V137 within GNASPromoterAvailabilityAcademic Institutions and Nonprofits only -
GoldenBraid 2.0 Kit
Plasmid Kit#1000000076PurposeModular cloning of multigenic DNA constructs in Plant Synthetic Biology applications. 95 GoldenBraid cloning compatible plasmids, containing standard basic parts and transcriptional unitsDepositorAvailabilityAcademic Institutions and Nonprofits only -
AIMTOR T757
Plasmid#140828PurposeAIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cellsDepositorInsertAIMTOR T757 (ULK1 Human)
UseTagsNES Nuclear export signalExpressionMammalianMutationThe insert comprises only a small sequence of hum…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCDH-SFFV-GLuc-CreERT2-mCherry
Plasmid#178185Purposethe plasmid promotes the constitutive expression of CreERT2 to enable intracellular recombination, Gaussia Luc can be used for in vivo imaging and mCherry is used as a marker for ex vivo analyses.DepositorInsertsGLuc
CreERT2
mCherry
UseLentiviralTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pmiR-report-MYB-WT
Plasmid#141114PurposeReporter plasmid of WT MYB in mammalian cells.DepositorInsertCMYB (MYB Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pmiR-report-MYB-mut
Plasmid#141115PurposeReporter plasmid of mutant MYB in mammalian cells.DepositorInsertMYB (MYB Human)
UseLuciferaseTagsExpressionMammalianMutationA to T mutations at m6A sitesPromoterAvailabilityAcademic Institutions and Nonprofits only -
2XSmBiT-SARS-CoV-2 S1
Plasmid#173430Purposeprotein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 FcDepositorInsert2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)
UseLuciferaseTagshuman IgG1 FcExpressionMammalianMutationfurin cleavage site RRAR to GSASPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pMIR-REPORT-MYC CDS_WT
Plasmid#108924PurposeMYC mRNA CDS region with wild type m6A motifs was inserted into pMIR-REPORT vectorDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pEXSISERS_BSD_FRT-PuroR
Plasmid#177867PurposeCloning Backbone for Blasticidin-S-deaminase-based EXSISERS containing FRT-sites flanked PuroR cassetteDepositorInsertBSD-based EXSISERS
UseCRISPR, Luciferase, Synthetic Biology, and Unspec…TagsOLLASExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Luc.Cre empty vector
Plasmid#20905DepositorTypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-FTO promoter_WT
Plasmid#108922PurposeThe core promoter region of FTO was inserted into pGL3 basic vectorDepositorInsertFTO (FTO Human)
UseTagsNAExpressionMammalianMutationNAPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGL3-basic-FTO promoter_Mut
Plasmid#108923PurposeThe core promoter region of FTO with mutant CEBPA binding sites was inserted into pGL3 basic vectorDepositorInsertFTO (FTO Human)
UseTagsNAExpressionMammalianMutationNAPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNOC-NLUC
Plasmid#98141PurposeGateway compatible construct for N' terminal fusion or promoter driven NanoLuciferase reporter, hygromycin resistance cassetteDepositorTypeEmpty backboneUseAlgae, nannochloropsisTagsHAExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Ubc.IkBSR-Flag Pgk.Cre
Plasmid#22503DepositorInsertIkBSR
UseCre/Lox and LentiviralTagsFlagExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
C231-E02: attB2r-NanoLuc-attB3
Plasmid#162901PurposeGateway attB2r/attB3 entry clone for construction of C-terminal NanoLuc fusion proteinsDepositorInsertNanoluciferase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pNH-NanoLuc
Plasmid#173075PurposeExpression vector for E. coli producing target protein with N-terminal Nanoluciferase fusionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLenti-mU6-Luc2GO-PGK-Neo
Plasmid#136905PurposeLentivirus for base editing activatable Luciferase2 expression in mammalian cells. All in one vector with sgLuc2GO.DepositorInsertLuc2CyGo
UseLentiviralTagsExpressionMutationATG>ACGPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
L2125-FRB-GPA-NLuc-CyOFP1
Plasmid#222885PurposePositive control receptor chain for BRET signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase, 10 GS linker, CyOFP1.DepositorInsertFRB-GPA-20GS-NanoLuc-10GS-CyOFP1
UseTagsMycExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 3941 - 3960 of 3967 results