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153,918 results

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pJF143.J3

Plasmid

#153028

Purpose

J3-mRFP1

Depositor

Jesse Zalatan

Article

Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

Insert

J3-mRFP1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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GluA1 (px330:2-guideCas9)

Plasmid

#169417

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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PB-CuO-V5 CASC3 siRes 1-480 WT

Plasmid

#158541

Purpose

For PiggyBac-mediated integration of V5-tagged siRNA-resistant CASC3 1-480

Depositor

Niels Gehring

Article

Gerbracht et al Nucleic Acids Res. 2020 Jul 4. pii: 5867

Insert

CASC3; BTZ; MLN51 (CASC3 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Amino acids 1-480, C-terminal deletion

Promoter

CMV

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluA1 TKIT donor

Plasmid

#169418

Purpose

Donor DNA for TKIT knockin of SEP to GluA1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEX-GFP-hLC3WT

Plasmid

#24987

Depositor

Isei Tanida

Article

Tanida et al Autophagy. 2008 Jan 1. 4(1):131-4.

Insert

a wild type human MAP1-LC3B fused with EGFP (MAP1LC3B Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pLVX-puro-cOVA

Plasmid

#135073

Purpose

Expresses chicken cytoplasmic ovalbumin in lentiviral vector with puromycin resistance

Depositor

Maria Castro

Insert

cOVA (OVAL Chicken)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

Plasmid

#48295

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

His6

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pCherry.90 alpha

Plasmid

#108222

Purpose

Mammalian expression vector for mCherry fusion to human Hsp90 alpha

Depositor

Didier Picard

Article

Lev et al Immunity. 2008 Jun;28(6):787-98. doi: 10

Insert

mCherry-Hsp90a (HSP90AA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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G-CaMP3

Plasmid

#22692

Purpose

a single-wavelength GCaMP2-based genetically encoded calcium indicator

Depositor

Loren Looger

Article

Tian et al Nat Methods. 2009 Dec;6(12):875-81.

Insert

G-CaMP3

Use

Tags

Expression

Mammalian

Mutation

N363D mutation (increased fluorescence)

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1_SDHB

Plasmid

#177981

Purpose

lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

sgRNA targeting SDHB (SDHB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

N/A

Promoter

U6

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-hGFAP-mCherry-shPtbp1

Plasmid

#178589

Purpose

Expression of mCherry and a shRNA against Ptbp1 under the human GFAP promoter

Depositor

Chun-Li Zhang

Article

Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

Insert

miRE-Ptbp1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-FLEX-rc [ChrimsonR-GFP]

Plasmid

#84480

Purpose

AAV-mediated expression of ChrimsonR-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

ChrimsonR-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

human synapsin promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

Plasmid

#160458

Purpose

Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

Depositor

April Pyle

Article

Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

Inserts

PAX7 Left/5' Homology Arm (PAX7 Human)
PAX7 Right/3' Homology Arm (PAX7 Human)

Use

Insertion of a fluorescent reporter through homol…

Tags

Expression

Mutation

Promoter

Available since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-puro

Plasmid

#39481

Purpose

Tetracycline inducible 3rd generation lentiviral vector

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

V5 and 6XHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pUF1-dCas9-GCN5

Plasmid

#122509

Purpose

Combining with the specific sgRNA sequence to activate of the Plasmodium falciparum endogenous gene transcription, through the increasing of H3K9 acetylation.

Depositor

Lubin Jiang

Article

Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

Insert

dCas9-GCN5

Use

CRISPR

Tags

3*FLAG

Expression

Mutation

dCas9(D10A,H840A)

Promoter

PfHsp86

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_PRPF19:1-504

Plasmid

#211132

Purpose

Mammalian expression of full-length human PRPF19. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

PRPF19:M1-L504 (PRPF19 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pJYS2cm_crtYf

Plasmid

#85543

Purpose

Constitutive transcription of crRNA of crtYf, Chloramphenicol resistance

Depositor

Sheng Yang

Article

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Insert

crRNA of crtYf

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-E2-Crimson

Plasmid

#111252

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with E2-Crimson

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

E2-Crimson

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pc

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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AttB _EGFP

Plasmid

#171597

Purpose

Strong EGFP fluorescence from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Insert

EGFP

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTipi2.1

Plasmid

#225877

Purpose

Allows for expression of recombinant antibodies

Depositor

Institute for Protein Innovation (IPI)

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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