Skip to main content
Addgene
Showing: 3921 - 3940 of 22262 results

  2. pETDuet-1 TIM9,10

    Plasmid
    #170280
    Purpose
    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Paul Schanda
    Article
    Weinhaupl et al Cell. 2018 Nov 15;175(5):1365-1379.e25.
    Insert
    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    170280-plasmid-map-sequence-id-334954

    Enlarge

  3. pICE-RNaseHI-D10R-E48R-NLS-mCherry

    Plasmid
    #60367
    Purpose
    Plasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.
    Depositor
    Patrick Calsou, Sébastien Britton
    Article
    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904
    Insert
    RNase HI-D10R-E48R
    Use
    Tags
    NLS from SV40 T antigen and mCherry
    Expression
    Mammalian
    Mutation
    D10R+E48R; catalyticaly inactive
    Promoter
    CMV-tet
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60367-plasmid-map-sequence-id-456413

    Enlarge

  5. mitoTALECD for_-20G_1333CN

    Plasmid
    #186200
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted 20th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186200-plasmid-map-sequence-id-368306

    Enlarge

  6. mitoTALECD for_ -13G_1397CN

    Plasmid
    #186199
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted -13th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1397C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1397N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186199-plasmid-map-sequence-id-368290

    Enlarge

  7. mitoTALECD for_ -6G_1333CN

    Plasmid
    #186198
    Purpose
    For genome editing (base editing) of Arabidopsis mitochondria, targeted -6th G to A of RNA editing site of ATP1 by OTP87
    Depositor
    Shin-ichi Arimura
    Article
    Nakazato et al Proc Natl Acad Sci U S A. 2022 May 17;11
    Inserts
    mitochondrial targeted TALE left hand with 1333C of Cytidine Deaminase (mitoTALECD)
    mitochondrial targeted TALE right hand with 1333N of Cytidine Deaminase (mitoTALECD)
    Use
    Synthetic Biology; Ti plasmid for plant transform…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    186198-plasmid-map-sequence-id-368288

    Enlarge

  8. pWPI-mPAX4

    Plasmid
    #114306
    Purpose
    Constitutive expression of the Pax4 trancription factor
    Depositor
    Patrick Salmon
    Insert
    PAX4 (Pax4 Mouse)
    Use
    Lentiviral
    Tags
    c-myc epitope, 6xHis tag
    Expression
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114306-plasmid-map-sequence-id-220634

    Enlarge

  9. pMLLCK-K112503

    Plasmid
    #26176
    Depositor
    Christopher Anderson
    Insert
    C terminal myc tag
    Use
    Synthetic Biology; 2ab assembly vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 23, 2012
    Availability
    Academic Institutions and Nonprofits only
    26176-plasmid-map-sequence-id-12297

    Enlarge

  18. pBiT3.1-N_DDB1:1-1140

    Plasmid
    #211147
    Purpose
    Mammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. N-terminal HiBiT tag.
    Depositor
    Cheryl Arrowsmith
    Article
    Ackloo et al bioRxiv 2024.03.03.583197
    Insert
    DDB1:M1-H1140 (DDB1 Human)
    Use
    Tags
    MVSGWRLFKKISGSSGGSSG
    Expression
    Mammalian
    Mutation
    loop deletion I396-D689
    Promoter
    CMV
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    211147-plasmid-map-sequence-id-425951

    Enlarge

  20. pE6c

    Plasmid
    #17466
    Purpose
    modified pENTR vector for C-terminal EYFP tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    EYFP
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    Promoter
    Available since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17466-plasmid-map-sequence-id-8634

    Enlarge

Showing: 3921 - 3940 of 22262 results