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Plasmid#186621PurposeEncodes Stim1 with C-terminal domain deletion fused to YFPDepositorInsertStromal Interaction Molecule 1 (STIM1 Human)
UseTagsYFPExpressionMammalianMutationDeletion of C-terminal domain 601 to 685PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-RHOA_sgRNA
Plasmid#183877PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.DepositorInsertRHOA sgRNA spacer (RHOA Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK3_009
Plasmid#123724PurposeEncodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK systemDepositorInsertPHYB-VP16-NLS
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-6
Plasmid#172771PurposeBacterial expression of anti-mCherry nanobody LaM-6, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-6
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK0_015
Plasmid#123930PurposeEncodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertPB Destination - Insulator + tagBFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT
Plasmid#140205PurposeProof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.DepositorInsertsmCherry
sgRNA E1
UseCRISPR and Synthetic Biology; Proof-of-concept te…TagsExpressionMutationG174DPromoterPelcA and U3Available sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK0_017
Plasmid#123932PurposeEncodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertBxBI attB tagBFP destination vector
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB2899(X-2 MarkerFree)
Plasmid#73271PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-2 (Chr X: 194944..195980)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro SCRIB P305L
Plasmid#88887PurposeSCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backboneDepositorInsertSCRIB (SCRIB Human)
UseRetroviralTagsExpressionMutationProline to Leucine mutation @ 305th amino acidPromoterMSCV promoterAvailable sinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pONSY-CoNMM:mCherry
Plasmid#111878PurposeThis plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.DepositorInsertCapsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry
UseCapsaspora owczarzakiTagsmCherryExpressionMutationPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKD068
Plasmid#136471PurposeSSB-GFP IDL fusion expression plasmidDepositorInsertSSB-GFP (ssb E. coli)
UseTagssuperfolder GFPExpressionBacterialMutationsuperfolder GFP inserted between Phe148 and Ser149PromoterT7 promoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK3_020
Plasmid#123735PurposeEncodes rapamycin and tamoxifen activated split-dCAS9 (C-terminal) as a Type 3 part to be used in the MTK systemDepositorInsertERT2-fkbp-spdCa9(205-1368)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CytERM-mAvicFP1
Plasmid#129510PurposeExpresses CytERM-mAvicFP1 in mammalian cells (for OSER assay)DepositorInsertCytERM-mAvicFP1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-DNMT3A PWWP
Plasmid#59696PurposeContains histone modification interacting domain (DNMT3A PWWP)DepositorInsertDNMT3A PWWP (Dnmt3a Mouse)
UseTagsExpressionBacterialMutationPromotertac promoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-27
Plasmid#172752PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-27
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GP-2-CBX7 Chromo
Plasmid#59695PurposeContains histone modification interacting domain (CBX7 Chromo)DepositorInsertCBX7 Chromo (CBX7 Human)
UseTagsGSTExpressionBacterialMutationPromotertac promoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB2903(XI-2 MarkerFree)
Plasmid#73275PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-shGrm2-2344
Plasmid#120725PurposeLentiviral vector that expresses GFP and an shRNA targeting Grm2 (in pLL3.7)DepositorInsertGrm2 (Grm2 Rat)
UseLentiviral and RNAiTagsGFPExpressionMammalianMutationPromotermouse U6Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-shGrm2-972
Plasmid#120724PurposeLentiviral vector that expresses GFP and an shRNA targeting Grm2 (in pLL3.7)DepositorInsertGrm2 (Grm2 Rat)
UseLentiviral and RNAiTagsGFPExpressionMammalianMutationPromotermouse U6Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1 ASA
Plasmid#13479DepositorInsertMKP-1ASA (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationChanged Arginine 53 to Alanine, Changed Arginine…PromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only