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Plasmid#102605PurposeMammalian expression of cGAS fused to PSTCD tagDepositorInsertcGAS (CGAS Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 MDM2 S166D S186D
Plasmid#16236DepositorInsertMDM2 (MDM2 Human)
UseTagsExpressionMammalianMutationSer166 and Ser186 replaced by AspPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-Flag-RNF169
Plasmid#74243PurposeExpresses RNF169 in mammalian cellsDepositorInsertRNF169 (RNF169 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5 FRT/TO Plk4-mCherry
Plasmid#80269Purposemammalian expression plasmid for Plk4-mCherryDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Cdc42(T17N)
Plasmid#12601DepositorInsertEGFP-Cdc42 (T17N) (CDC42 Human)
UseTagsEGFPExpressionMammalianMutationT17NPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphasS-RLuc8
Plasmid#140980PurposeEncodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphasS-RLuc8
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-HDAC6.ΔBuz-FLAG
Plasmid#30484DepositorInsertHDAC6 (HDAC6 Human)
UseTagsFLAGExpressionMammalianMutationDeletion of the C-terminal Ubiquitin binding doma…PromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 deltaN89 Beta-catenin
Plasmid#19288DepositorInsertbeta-catenin (CTNNB1 Human)
UseTagsFlagExpressionMammalianMutationN-terminal truncation, deleted aa 1-89PromoterAvailable sinceSept. 8, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hAsCpf1(TATV) (pY220)
Plasmid#89353PurposeExpresses humanized AsCpf1 variant that recognizes TATV PAMsDepositorInsertCpf1, RVR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K548V/N552RPromoterCMVAvailable sinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/V5 hsa-mir-192
Plasmid#26311DepositorInserthsa-mir-192 (MIR192 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/V5-His Snk/Plk2
Plasmid#16015DepositorInsertSnk/Plk2 (PLK2 Human)
UseTagsV5-HisExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-Cre
Plasmid#167939Purposehuman CD81-TEV cleavage site-CreDepositorInserthuman CD81 (CD81 Human)
UseTagsCre recombinase and TEV protease cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-PhoCl2f-mCherry
Plasmid#164048PurposeOptogenetic control of protein localization by PhoCl2fDepositorInsertNES-PhoCl2f-mCherry
UseTagskozak sequenceExpressionMammalianMutationPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NOD1-EGFP
Plasmid#131206Purposemammalian expression, tet RDepositorInsertNOD1 (NOD1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-NES-ZapCV2 (cpV143)
Plasmid#112060PurposeGenetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)DepositorInsertNES-ZapCV2
UseTagsNuclear Export Signal (NES) derived from HIV-1ExpressionMammalianMutationECFP gene is truncated 36 bp at 3' end. Venu…PromoterCMVAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SARS-CoV-2-S (Wuhan)
Plasmid#215812PurposeExpresses SARS-CoV-2 Spike protein in mammalian cellsDepositorInsertSARS-CoV-2 Spike (S )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP
Plasmid#194685PurposeCMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFPDepositorInsertCaprola_09-mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1(T17N)
Plasmid#13721DepositorInsertEGFP-Rac1(T17N) (RAC1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-JEDI-2P
Plasmid#179458PurposeGenetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)DepositorInsertJEDI-2P
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.0_LynLAMA-G97 IRES EGFP
Plasmid#130711PurposePlasmid encoding outer inner membrane localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFPDepositorInsertLyn11-SNAP-Tag-LAMA-G97 IRES EGFP
UseTagsLyn11 and SNAP-TagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only