Showing: 3901 - 3920 of 3967 results
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Plasmid#186770PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRsDepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::sNluc0
Plasmid#186771PurposeAn mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRsDepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::RPL15::hNluc
Plasmid#186772PurposeAn mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRsDepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pNHT7::YB1::hNluc
Plasmid#186774PurposeAn mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRsDepositorInsertNanoluciferase
UseTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pGAPDH::sNluc1
Plasmid#187221PurposeA plasmid containing a planarian codon-optimized Nluc (sNluc1), driven by the planarian GAPDH promoter.DepositorInsertNanoluciferase
UseOverexpressionTagsExpressionMutationPromoterGAPDHAvailabilityAcademic Institutions and Nonprofits only -
pcDNA.3.1.ADRB2-NP
Plasmid#134376PurposeNanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.DepositorInsertADRB2-NP (ADRB2 Human)
UseTagsFlag, natural peptide of nanoluciferase, and sign…ExpressionBacterial and MammalianMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
TOPO mMecp2-NLucTom
Plasmid#159282PurposeTo tag endogenously and c-terminally mouse Mecp2 with two reporters: NanoLuciferase and TdTomato. It contains 2 homology arms from a castaneus background surrounding the STOP codon.DepositorInsertMecp2 (Mecp2 Mouse)
UseTagsNluciferase-P2A-TdTomatoExpressionMammalianMutationNluciferase-TdTomato fusionPromoterAvailabilityAcademic Institutions and Nonprofits only -
CRISPaint Gene Tagging Kit
Plasmid Kit#1000000086PurposeModular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tagsDepositorAvailabilityAcademic Institutions and Nonprofits only -
pSNRW-Z3-Nluc
Plasmid#174486PurposeRecombinant fusion protein gene combining IgG-Fc binding domain Z (3 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.DepositorInsertZ3-Nluc
UseTags6x Histidine tagExpressionBacterialMutationPromoterT7lac promoterAvailabilityAcademic Institutions and Nonprofits only -
pSNRW-Z5-Nluc
Plasmid#174487PurposeRecombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.DepositorInsertZ5-Nluc
UseTags6x Histidine tag (N terminal on insert)ExpressionBacterialMutationPromoterT7lac promoterAvailabilityAcademic Institutions and Nonprofits only -
pUltra-Chili-Luc
Plasmid#48688Purpose3rd generation Lentiviral vector with bi-cistronic expression of dtomato and luciferaseDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
CD44P pGL3
Plasmid#19122DepositorInsertCD44 promoter (CD44 Human)
UseNon viral cloning vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
BM00654
Plasmid#155339PurposeBinary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling FLuc. It constitutively expresses RLuc and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-RLuc-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-FLuc-POpto_LB
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphai1-RLuc8
Plasmid#140973PurposeEncodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphai1-RLuc8 (GNAI1 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA
Plasmid#104140PurposeExpresses TREAT-5'TOP reporter in mammalian cellsDepositorInsertRenilla luciferase
UseTags24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…ExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pTetON-DeAct-GS1
Plasmid#89454PurposeTet/Dox-inducible expression of EGFP-GS1 (Human Gelsolin 53-176) in mammalian cellsDepositorInsertDeAct-GS1 (GSN Human)
UseTagsEGFPExpressionMammalianMutationTruncation spanning cytoplasmic Gsn amino acids 5…PromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
pTetON-DHFRdd-SpvB; CMV-mCherry
Plasmid#89463PurposeTet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)DepositorInsertDeAct-SpvB (spvB Salmonella enterica)
UseTagsE. coli DHFRdd destabilization domain and mCherry…ExpressionMammalianMutationTruncation spanning SpvB amino acids 375-591 (mon…PromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
pMY-Ly6G-P2A-PuroR-HA-Rluc_miR
Plasmid#163340PurposeRetroviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase and expression of a Ly6G surface marker and an HA-tagged puromycin selection markerDepositorInsertLy-6G (Ly6g Mouse)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterLTRAvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-Ly6G-P2A-PuroR-HA-miR30
Plasmid#163359PurposeLentiviral vector for negative control of knockdown expressing a non-target miR against Renilla luciferase, with expression of a Ly6G surface marker and a puromycin selection marker.DepositorInsertLy-6G (Ly6g Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-Halo-hMRE11(wt)
Plasmid#208393PurposeTo generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection.DepositorInsertHuman MRE11 (MRE11 Human)
UseLuciferase and RetroviralTagsExpressionMammalianMutationH129NPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 3901 - 3920 of 3967 results