Addgene

We narrowed to 5,229 results for: hwa

Showing: 3901 - 3920 of 5229 results

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pGEX-4T1_GST-GBRPL2

Plasmid

#223730

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

GABARAPL2 (GABARAPL2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 2-116 only, with Glycine exposed

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_NIX(1-182aa)-THROMBIN-GST (WT)

Plasmid

#223733

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Use

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

aa 1-182 only

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (WT)

Plasmid

#223734

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

FUNDC1 soluble part (FUNDC1 Human)

Use

Tags

Thrombin cleavage-GST

Expression

Bacterial

Mutation

aa 1-50 only

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_GST-TEV-EGFP-ATG13

Plasmid

#223797

Purpose

Expression of recombinant protein for purification

Depositor

James Hurley, Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

ATG13 (ATG13 Human)

Use

Tags

GST-TEV-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)

Plasmid

#229742

Purpose

Expresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

LIR1 mutant WEVL -> AEVA

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)

Plasmid

#229743

Purpose

Expresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

LIR2 mutant WTFI -> ATFA

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-EF1A-HALO-hLRRK2 (LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA)

Plasmid

#229744

Purpose

Expresses Halo tagged human LRRK2 with LIR1 and LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

LRRK2 (LRRK2 Human)

Use

Tags

Halo

Expression

Mammalian

Mutation

LIR1+2 mutant WEVL -> AEVA / WTFI -> ATFA

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-R204A

Plasmid

#222196

Purpose

Bacterial expression of mouse PTER-R204A protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

R204A

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSecTaq2-hGas6-Myc-6xHis

Plasmid

#226523

Purpose

Expresses TAM Receptor Ligand human GAS6

Depositor

Raymond Birge, Sergei Kotenko

Article

Geng et al Front Immunol. 2017 Nov 10;8:1521. doi:

Insert

Human GAS6 (GAS6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pNK7190

Plasmid

#219763

Purpose

MoClo-compatible Level M for expression nnLuz, nnH3H, nnCPH in plants (N.benthamiana, BY2 cells)

Depositor

Karen Sarkisyan

Article

Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

Insert

nnLuz, nnH3H, nnCPH

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-H230F

Plasmid

#222195

Purpose

Bacterial expression of mouse PTER-H230F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H230F

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER-H26F

Plasmid

#222192

Purpose

Bacterial expression of mouse PTER-H26F protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Use

Tags

6xHis and Strep

Expression

Bacterial

Mutation

H26F

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST40-mPTER

Plasmid

#224864

Purpose

Mammalian expression mouse PTER protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

mouse PTER (Pter Mouse)

Use

Tags

C-term v5 and His tag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV_Puro_SFB_GNB1L

Plasmid

#224381

Purpose

Lenti plasmid for generating GNB1L expressing stable cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pX459_TELO2_gRNA

Plasmid

#224379

Purpose

Targets TELO2 for dTAG N terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

TELO2 (TELO2 Human)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pX459_GNB1L_gRNA

Plasmid

#224377

Purpose

Targets GNB1L for dTAG C terminal knock-in cell lines

Depositor

Junjie Chen

Article

Huang et al Mol Cell. 2023 Aug 3;83(15):2810-2828.e6

Insert

GNB1L (GNB1L Human)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CD28-GCN4-His6

Plasmid

#223585

Purpose

Protein purification of human CD28 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD28 (CD28 Human)

Use

Tags

GCN4, His6 and Twin-Strep, SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CTLA-4-GCN4-His6

Plasmid

#223582

Purpose

Protein purification of human CTLA4 with Twin-Strep, SNAP, GCN4 and 6xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CTLA4 (CTLA4 Human)

Use

Tags

GCN4, His6 and Twin-Strep, SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CD80 (I92R)-His10

Plasmid

#223580

Purpose

Protein purification of human CD80 (I92R) with Twin-Strep, SNAP and 10xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Use

Tags

His10 and Twin-Strep, SNAP

Expression

Mammalian

Mutation

I92R

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-CD80-His10

Plasmid

#223579

Purpose

Protein purification of human CD80 with Twin-Strep, SNAP and 10xHis tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Use

Tags

His10 and Twin-Strep, SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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