Addgene

We narrowed to 27,942 results for: eng

Showing: 3901 - 3920 of 27942 results

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pICH37431

Plasmid

#50306

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

signal peptide, RAmy3A (O. sativa)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH45089

Plasmid

#50254

Depositor

Sylvestre Marillonnet

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH42760

Plasmid

#50257

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, spm (Z. mays)

Use

Plant expression

Tags

Expression

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGGI000

Plasmid

#48863

Purpose

Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGE003

Plasmid

#48854

Purpose

Provides the At1g04880 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

At1g04880 terminator

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGD006

Plasmid

#48836

Purpose

Provides the SV40 nuclear localization signal as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

SV40 NLS

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGE002

Plasmid

#48840

Purpose

Provides the WUS terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS terminator

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGB006

Plasmid

#48823

Purpose

Provides an ER signal sequence as GreenGate N-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

signal sequence ER

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGGC014

Plasmid

#48827

Purpose

Provides GFP as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

GFP

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

A206K

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBiFC-VN173

Plasmid

#22010

Purpose

Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

VN173

Use

Tags

FLAG

Expression

Mammalian

Mutation

Venus residus 1-172

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pBiFC-VC155

Plasmid

#22011

Purpose

Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

VC155

Use

Tags

HA

Expression

Mammalian

Mutation

Changed Alanine 206 to Lysine

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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HC-M

Plasmid

#173481

Purpose

An rrnB P1-based GFP ATP reporter in E. coli

Depositor

Rahul Sarpeshkar

Article

Deng et al BMC Biol. 2021 May 17;19(1):101. doi: 10

Insert

GFP-mut2

Use

Synthetic Biology

Tags

ssrA degradation tag

Expression

Bacterial

Mutation

Promoter

rrnB P1

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBluescript-Evf1

Plasmid

#99479

Purpose

Subcloning of the Evf1 long non-coding RNA

Depositor

Jhumku Kohtz

Article

Feng et al Genes Dev. 2006 Jun 1;20(11):1470-84. Ep

Insert

Evf1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pSIBR4-Cre

Plasmid

#219435

Purpose

Theophylline_induced self_splicing intron_based riboswitch for the moderate expression of Cre recombinase

Depositor

Sheng Yang

Article

Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-

Insert

intron 4-Cre

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBE48

Plasmid

#174656

Purpose

CAPTURE method BAC origin of replication receiver plasmid

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBE45

Plasmid

#174655

Purpose

CAPTURE method receiver plasmid for Streptomyces heterologous expression

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pBE44

Plasmid

#174654

Purpose

CAPTURE method 15A origin of replication receiver plasmid

Depositor

Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pGGZ003

Plasmid

#48869

Purpose

Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible plant transformation vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Filamin-C-stFRET

Plasmid

#61107

Purpose

Expression of Filamin with C-term stFRET inserted to measure mechanical stress in real time

Depositor

Fred Sachs

Article

Meng et al FEBS J. 2008 Jun;275(12):3072-87. doi: 1

Insert

filamin-stFRET

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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pGGF003

Plasmid

#48844

Purpose

Provides a D-alanine resistance cassette as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

pMAS::D-AlanineR::tMAS

Use

Golden gate compatible cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge