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pST50Trc4-HISNDHFR
Plasmid#63994PurposePosition 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
5FAI (EMG1)
Plasmid#79519PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEMG1 (EMG1 Human)
UseTags6xHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4134207
Plasmid#152431PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4252903
Plasmid#153778PurposeMammalian expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;V5ExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133141
Plasmid#152404PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 17, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4258326
Plasmid#154793PurposeMammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 surface glycoprotein (Spike protein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;V5ExpressionMammalianMutationwtPromoterCMVAvailable sinceJuly 30, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133151
Plasmid#153581PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4133806
Plasmid#153713PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;S-tagExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAT11
Plasmid#112592PurposeUsed for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP.DepositorTypeEmpty backboneUseTags6His-MBPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc2-HISNDHFR
Plasmid#63958PurposePosition 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTags6xHis; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4252875
Plasmid#153789PurposeMammalian expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;3xFlagExpressionMammalianMutationwtPromoterCMVAvailable sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
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5UBB (LOC149281)
Plasmid#101257PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMETTL11B (METTL11B Human)
UseTags8xHis-Thrombin-SUMO-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
LMNB2
Plasmid#36896PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLMNB2 (LMNB2 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationcontains Laminb2 Ig-like domain, amino acid resid…PromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pST50Trc3-STRNDHFR
Plasmid#63978PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsStrep II (STR); TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
5VC8 (WHSC1)
Plasmid#101262PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertWHSC1 (NSD2 Human)
UseTags6xHis-TEVExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4133341
Plasmid#152285PurposeMammalian expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;V5ExpressionMammalianMutationwtPromoterCMVAvailable sinceSept. 25, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
ANKRA2
Plasmid#36904PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertANKRA2 (ANKRA2 Human)
UseTagsHis tag with TEV cleavageExpressionBacterialMutationamino acid residues 148-313 (ANK repeats)PromoterAvailable sinceJune 19, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pST50Trc3-FLAGNDHFR
Plasmid#63979PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneUseTagsFLAG; TEV cleavableExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only
