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Plasmid#202223PurposeLevel 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and tagBFP fluorescent markerDepositorInsertpBCC25, pBCC30, pBCC59, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV.cc81-mut
Plasmid#199745PurposeExpresses AAV2 Rep and AAV.cc81 CapDepositorInsertAAV.cc81 Cap
UseAAVTagsExpressionMammalianMutationmutated amino acids 586-592 in CapPromoterAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.cc84-mut
Plasmid#199746PurposeExpresses AAV2 Rep and AAV.cc84 CapDepositorInsertAAV.cc84 Cap
UseAAVTagsExpressionMammalianMutationmutated amino acids 586-592 in CapPromoterAvailable sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJUMP24_T24_pRham-ilvA-relE_RBS3_CB3
Plasmid#201534PurposeExpressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)DepositorInsertilvA-relE_RBS3_CB3
UseSynthetic BiologyTagsExpressionMutationrhaS W190GPromoterPrhaBAD (rhamnose)Available sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTE1069
Plasmid#186629PurposeSCB-GFP E. coli reporter plasmid. Encodes repressor ScbR and ScbAp promoter upstream of GFP.DepositorInsertsscbR
scbAp promoter
UseSynthetic BiologyTags6xArgExpressionBacterialMutationPromoterBBa_J23100 and scbApAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056H
Plasmid#183135PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056J
Plasmid#183136PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
1056K
Plasmid#183137PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJune 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSN526
Plasmid#184827PurposeExpresses human KIF5A(∆exon27) in insect cellsDepositorInsertKIF5A (∆exon27) (KIF5A Human)
UseTagsmScarlet-2xStrepIIExpressionInsectMutation∆exon27 form of human KIF5APromoterpolyhedrinAvailable sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
1137B=Hsp70Bb-Cas9
Plasmid#153284PurposeThe attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.DepositorInsertpHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
UseCRISPRTagseGFPExpressionInsectMutationPromoterDmel Hsp70BbAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Ub-VPS13D
Plasmid#176462Purposeexpressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13DDepositorInsertVPS13D (VPS13D Human)
UseTagsGFP-UbExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Ub-VPS13D N3521S
Plasmid#176463Purposeexpressing VPS13D patient mutant N3521S in mammalian cellsDepositorInsertVPS13D (VPS13D Human)
UseTagsGFP-UbExpressionMammalianMutationN3521S mutationPromoterCMVAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-NKD2S31A
Plasmid#174331Purposeencodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag.DepositorInsertHomo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)
UseTagsMycHisExpressionMammalianMutationchanged Serine 31 to AlaninePromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfPV-1.8k-Venus-WPRE
Plasmid#156398PurposeExpresses Venus under PV gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfSP-0.8k-Venus-WPRE
Plasmid#156397PurposeExpresses Venus under SP gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfSP-1.7k-Venus-WPRE
Plasmid#156396PurposeExpresses Venus under SP gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfSERT-0.5k-Venus-WPRE
Plasmid#156395PurposeExpresses Venus under SERT gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfvAChT-1.1k-Venus-WPRE
Plasmid#156390PurposeExpresses Venus under vAChT gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTYF-super-mfCCK-3.9k-Venus-WPRE
Plasmid#156391PurposeExpresses Venus under CCK gene promoterDepositorInsertsVenus
Gal4p65
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Opie2-PuroR[dsxM]
Plasmid#131616PurposeExpresses a functional PuroR only in males and dsRed in both genders.DepositorInsertsUseTagsExpressionInsectMutationPromoterOpie2 and hr5Ie1Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only