Skip to main content
Addgene
Showing: 3881 - 3900 of 3910 results

  2. pGEX2T-TEV CHMP1B 186-199 L188A/L192A

    Plasmid
    #108288
    Purpose
    Expresses residues 186-199 with L to A mutations at residues 188 and 192 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-286
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108288-plasmid-map-sequence-id-245273

    Enlarge

  3. pGEX2T-TEV CHMP1B 186-199 L192A/L195A

    Plasmid
    #108290
    Purpose
    Expresses residues 186-199 with L to A mutations at residues 192 and 195 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-287
    Depositor
    Wesley Sundquist
    Article
    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-
    Insert
    CHMP1B (CHMP1B Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    changed L 188 to A, changed L 192 to A
    Promoter
    Available since
    April 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    108290-plasmid-map-sequence-id-245275

    Enlarge

  4. pME-mCherry-PA-Rac1

    Plasmid
    #169042
    Purpose
    Middle entry vector for Gateway cloning that contains an mCherry fused with an optimized photoactivatable Rac1 gene insert
    Depositor
    Paola Arlotta
    Article
    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d
    Insert
    PA-Rac-1 (RAC1 Human, Avena sativa (oat))
    Use
    Gateway entry cloning vector
    Tags
    6X His and Fused with mCherry
    Expression
    Mutation
    Rac1 starts at I4 and contains mutations Q61L, E9…
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    169042-plasmid-map-sequence-id-341784

    Enlarge

  6. pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct

    Plasmid
    #156433
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse Cdca5 (SORORIN) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA GGGATGCCCGTCATTAAGTG
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    SORORIN, AID (Cdca5 Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156433-plasmid-map-sequence-id-310157

    Enlarge

  13. Fire Lab C. Elegans Kit

    Plasmid Kit
    #1000000001
    Purpose
    Worm (C. elegans) vector set comprised of lacZ/GFP fusions, exon and intron GFP tagging vectors, RNAi controls, and hairpin sequences for testing the ability of promoters to in vivo trigger RNAi
    Depositor
    Andrew Fire
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  14. Minimal Antibiotic Resistance Platform (ARP)

    Plasmid Kit
    #1000000143
    Purpose
    Used for the general dereplication of antibiotics in natural product extracts. Plasmids constitutively express individual resistance genes that target the most commonly found antibiotics.
    Depositor
    Gerard Wright
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  15. pCut plasmid toolkit

    Plasmid Kit
    #1000000118
    Purpose
    Cas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.
    Depositor
    Aindrila Mukhopadhyay
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only

  16. ScEnSor Kit

    Plasmid Kit
    #1000000215
    Purpose
    This three-step (Build - Transform - Assess) Saccharomyces cerevisiae kit is comprised of two modules (Engineering and Biosensor) that can be used independently or in combination.
    Depositor
    Lisbeth Olsson
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  17. MXS Chaining Kit

    Plasmid Kit
    #1000000064
    Purpose
    MXS-chaining system for assembly of constructs optimized for synthetic biology applications in mammalian systems. 14 different fluorescent proteins, 10 mammalian promoters/enhancers, 3 polyA signals
    Depositor
    Pierre Neveu
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. MoClo Tool Kit

    Plasmid Kit
    #1000000044
    Purpose
    Eukaryotic multigene construct assembly in 3 steps using modular cloning (MoClo); Has 95 empty standardized genetic modules: promoters, 5' UTRs, signal peptides, coding sequences, and terminators
    Depositor
    Sylvestre Marillonnet
    Available since
    April 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. MoClo Plant Parts II and Infrastructure Kit

    Plasmid Kit
    #1000000135
    Purpose
    Compatible with the Modular Cloning (MoClo) system for hierarchical assembly of plant multigene constructs, with new phytobricks and vectors to connect Modular Cloning and Gateway Cloning platforms.
    Depositor
    Johannes Stuttmann
    Available since
    Sept. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. GoldenBraid 2.0 Kit

    Plasmid Kit
    #1000000076
    Purpose
    Modular cloning of multigenic DNA constructs in Plant Synthetic Biology applications. 95 GoldenBraid cloning compatible plasmids, containing standard basic parts and transcriptional units
    Depositor
    Diego Orzaez
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 3881 - 3900 of 3910 results