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Plasmid#135929PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).DepositorInsertmCherry fused to TNFSF9
UseTransposonTagsmCherryExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C-GFP
Plasmid#130975PurposeExpression of 6His-KIF1C-GFP in insect cells for protein purification.DepositorInsertKIF1C-GFP (KIF1C Human)
UseTags6His and GFPExpressionInsectMutation5 silent mutations that make this construct RNAi ā¦PromoterPolyhedrinAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
sgTrack-mCherry
Plasmid#114013PurposeEmpty sgRNA expression vector with co-expression of mCherry reporterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-dCas9-VP64-6xHis
Plasmid#62935PurposeExpression of dCas9-VP64-6xHis in bacterial cellsDepositorInsertdCas9-VP64
UseTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A and H840A amino acid changes render Cas9 nucā¦PromoterT7Available sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisB-TRF2
Plasmid#50488PurposeE. Coli N-term HexHis Tagged Protein Expression VectorDepositorInsertTRF2 (TERF2 Human)
UseTags6x His, T7 tag, and TEV protease siteExpressionBacterialMutationContains amino acids 3-500PromoterpTrcAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCasPA
Plasmid#113347PurposeBacterial expression of Cas9 nuclease and Ī»-Red system in Pseudomonas aeruginosaDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt1
Plasmid#49186PurposecDNA of Akt1 with myristyolation signal in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationPromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
CENP-B mCherry
Plasmid#45219DepositorInsertCENP-B mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2013AvailabilityAcademic Institutions and Nonprofits only -
GLP1R-DuET
Plasmid#213253PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGLP1R (GLP1R Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1-6xflag-RT3-3xHaloTag(MtoL)-H2B
Plasmid#106309PurposeThe plasmid utilizes RT3 sequence to minimize the expression of 3xHaloTag (Methionine mutated to Leucine)-H2B for long-term H2B single-molecule imaging.DepositorInsert6xflag-RT3-3xHaloTag(MtoL)-H2B (H2BC3 Human)
UseTags6xflag-3xHaloTag(MtoL)ExpressionMammalianMutationPromoterEF1Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CAT
Plasmid#173943PurposeBacterial expression of isolated PARP1 CAT domainDepositorInsertPARP1-CAT (PARP1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV-SNAP-hCB1_IRES_EGFP
Plasmid#223505PurposeExpression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection markerDepositorInsertSNAP-tag-CNR1 and EGFP (CNR1 Synthetic, Human)
UseTagsSNAP-tagExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pGL4.27_ARE/NRF2-SPE
Plasmid#177775PurposeLuciferase reporter for ARE/NRF2DepositorInsertNRF2 (NFE2L2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-NIK-HA
Plasmid#27554DepositorInsertNIK (MAP3K14 Human)
UseTagsHAExpressionMammalianMutationNIK starts at amino acid 3; C52R and R139H mutatiā¦PromoterCMVAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL4.R_TRE_minCMV
Plasmid#211518PurposeDual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expressionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterSV40 and minCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP
Plasmid#221233PurposeExpress sgRNA targeting HEK3 loci with nCas9(D10A)DepositorInsertSpCas9(D10A)-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH3.3_Cre-MS2x12
Plasmid#205559PurposeExpress Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertCre
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
spMSP1D1
Plasmid#173482PurposeExpresses spMSP1D1 in Bl21DepositorInsertspMSP1D1
UseTagsHistagExpressionBacterialMutationPromoterAvailable sinceSept. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
HDM_VSV_G
Plasmid#204156PurposeVSV-G expression plasmidDepositorInsertVSV-G
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only