We narrowed to 4,536 results for: hwa

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pcDNA FZD5_4CRDlinker

Plasmid

#184264

Purpose

Expresses FLAG tagged FZD5 swapped with FZD4 CRDlinker domain

Depositor

Hee-Jung Choi

Article

Ko et al Commun Biol. 2022 May 5;5(1):421. doi: 1

Insert

FZD5_4CRDlinker (FZD5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Replaced FZD5 aa27-221 with FZD4 aa41-203

Available Since

Aug. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pQdCas9-sggfp

Plasmid

#236184

Purpose

The plasmid pQdCas9-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the gfp gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

Quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene

Promoter

Quorum sensing promoter

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pQdCas12a.sggfp(A)

Plasmid

#236038

Purpose

The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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shRNA_circSMAD2_2

Plasmid

#215222

Purpose

Supression of shcircSMAD2(2,7)_2 expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

circSMAD2 shRNA 2 (SMAD2 Human)

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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shRNA_SMAD2_2

Plasmid

#215203

Purpose

Supression of shSMAD2 expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

SMAD2 shRNA 2 (SMAD2 Human)

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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shRNA_SMAD2_1

Plasmid

#215204

Purpose

Supression of shSMAD2_1 expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

SMAD2 shRNA 1 (SMAD2 Human)

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pACB143

Plasmid

#222214

Purpose

For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag

Depositor

Gregg Beckham

Article

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

vanB(A24P)

Tags

N-terminal, thrombin-cleavable 6x His tag

Expression

Bacterial

Mutation

Changed Alanine 24 to Proline

Promoter

T7

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-p62 var2

Plasmid

#204576

Purpose

Expresses 3xFLAG-p62 var in mammalian cells

Depositor

Masaaki Komatsu

Article

Kageyama et al Mol Cell Biol. 2018 Jan 16. pii: MCB.006

Insert

p62 (Sqstm1 Mouse)

Expression

Mammalian

Mutation

None

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET28b-MBP-bhaB5

Plasmid

#167812

Purpose

Expresses His6- and MBP-tagged BhaB5 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaB5

Tags

His6, MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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SNAP-Kindlin

Plasmid

#186136

Purpose

For expression of recombinant Snap-labeled kindlin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

FERMT2 (FERMT2 Human)

Tags

His6-sumo

Expression

Bacterial

Promoter

T7

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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Lck

Plasmid

#186130

Purpose

For expression of recombinant Lck in SF9 cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

Lck (Lck Mouse)

Tags

His6

Expression

Insect

Mutation

A1687T

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hSEC61B-C-PEN2

Plasmid

#183648

Purpose

Lentiviral expression of a ER-residential PEN2.

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and SEC61B (PSENEN, SEC61B Human)

Use

Lentiviral

Tags

HA

Mutation

WT

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hPEN2 2A

Plasmid

#183640

Purpose

Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).

Depositor

Sheng-cai Lin

Article

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 (PSENEN Human)

Use

Lentiviral

Tags

HA

Mutation

residues 35 and 40 of PEN2 mutated to alanine

Promoter

CMV

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJH202

Plasmid

#172150

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp 32A; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*); 32A pylS variant

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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MSCVhyg-HA-FLAG_Ubc9_C93A

Plasmid

#164937

Purpose

Expresses FLAG & HA tagged human Ubiquitin Conjugating Enzyme 9 (UBC9) _C93A mutant cDNA

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

human UBC9 (UBE2I Human)

Use

Retroviral

Tags

FLAG & HA tags

Promoter

PGK promoter

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L-His-SpyTag-XMod-Doc(WT)

Plasmid

#157675

Purpose

Expression of XMod-Doc wild type with His6x tag and SpyTag.

Depositor

Michael Nash

Article

Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

Insert

XMod-Doc Wild type with Histag and SpyTag

Tags

Histag and SpyTag

Expression

Bacterial

Promoter

T5

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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KG#80

Plasmid

#110875

Purpose

Expresses the C. elegans acy-1 cDNA in body wall muscle

Depositor

Kenneth Miller

Article

Reynolds et al Genetics. 2005 Feb;169(2):651-70. doi: 1

Inserts

myo-3 promoter
acy-1 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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P0050::rpaAD53A

Plasmid

#101831

Purpose

drives expression of rpaA D53A variant from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

RpaA D53A with p0050 promoter (+ gentamicin resistance casette)

Use

Cloning vector

Mutation

D53 mutated to A

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pTCW022

Plasmid

#83532

Purpose

pheromone mediated expression of UBiC, ARO4, and TKL1

Depositor

Claudia Vickers

Article

Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

Insert

pFUS1J2-UBiC-CYC1t-pFUS1J2-ARO4*-CYC1t-pFUS1J2-TKL1-CYC1t

Expression

Yeast

Promoter

pFUS1J2

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-dSestrin (CG11299-PD)

Plasmid

#73675

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn (variant D) (Sesn Fly)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only