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Plasmid#187463PurposeExpresses red acetylcholine indicator AchLightR in neuronal cellsDepositorInsertAchLightR
UseAAVTagsIgκ leaderExpressionMutationPromoterhuman Synapsin-1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Frb-v1
Plasmid#201923PurposeE. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.DepositorInsertsFrbA
FrbB
FrbC
FrbD
FrbE
UseTagsExpressionBacterialMutationPromoterT7 (modified), T7 (modified, moderate), T7 (modif…Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6XHis-SUMO-SIRT2
Plasmid#102622PurposeExpresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purificationDepositorInsertSIRT2 (SIRT2 Human)
UseTags6XHis-SUMOExpressionBacterialMutationDeleted amino acids 1-37 and 357-389PromoterAvailable sinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiGuideFE-Puro
Plasmid#170069PurposeExpresses S. pyogenes CRISPR chimeric RNA element (with F+E modifications) with customizable gRNA from U6 promoter and puromycin resistance from EFS-NS promoterDepositorInsertCas9 guide RNA scaffold with the F+E scaffold modification
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site
Plasmid#172508PurposeEncodes the mature DisCas7-11 DR along with a golden gate site for spacer cloningDepositorInsertpU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-Flag-c-Myc
Plasmid#102625PurposeExpresses c-Myc in mammalian cellsDepositorInsertMYC (MYC Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJan. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mGM-CSF
Plasmid#74465Purposeexpression of mGM-CSF in mammalian cellsDepositorInsertgranulocyte-monocyte colony-stimulating factor (Csf2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eGFP-mPlum-HA
Plasmid#200009PurposeDonor vector with double fluorescence system for targeted integration using CRISPR/Cas9. Expresses 5´HA--eGFP&PuroR--3´HA--mPlum. Distinguish targeted from random integration by fluorescence.DepositorInserteGFP, mPlum
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-FKBP-TPD54
Plasmid#172454PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal GFP-FKBP tagDepositorInsertTPD54 (TPD52L2 Human)
UseTagsGFP-FKBPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
TetO-Ascl1-puro
Plasmid#97329PurposeGenerating lentiviruses to express Ascl1 and puromycin resistanceDepositorInsertAscl1 (Ascl1 Mouse)
UseLentiviralTagsExpressionMutationPromoterTET-ONAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightG
Plasmid#187179PurposeExpresses green norepinephrine indicator nLightG in neuronal cellsDepositorHas ServiceAAV9InsertnLightG
UseAAVTagsFlag tagExpressionMutationPromoterhuman Synapsin-1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_AchLightG
Plasmid#187464PurposeExpresses green acetylcholine indicator AchLightG in neuronal cellsDepositorInsertAchLightG
UseAAVTagsIgκ leaderExpressionMutationPromoterhuman Synapsin-1Available sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
DLX2-hygro
Plasmid#97330PurposeGenerating lentiviruses to express Dlx2 and hygromycin resistanceDepositorInsertDLX2 (Dlx2 Mouse)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
SFFV-IKZF3-CTERM-GFP
Plasmid#185769PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, IKZF3 minidegronExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
SFFV-DTAG-NTERM-GFP
Plasmid#185760PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
SFFV-HALO-CTERM-GFP
Plasmid#185767PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, HaloExpressionMutationnonePromoterSFFVAvailable sinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/cyto-iAspSnFR
Plasmid#201394PurposeCMV overexpression of cyto-iAspSnFR in mammalian cell linesDepositorInsertcyto-iAspSnFR
UseTagsExpressionMammalianMutationgltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…PromoterCMVAvailable sinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJ075_hsCRBN
Plasmid#124214PurposeInsect cell expression vector for His6 tagged hsCRBNDepositorInsertCereblon (CRBN Human)
UseTagsHis6-TEVExpressionInsectMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
SFFV-DTAG-CTERM-GFP
Plasmid#185765PurposeDegron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloningDepositorInsertGFP
UseLentiviralTagsV5, dTAGExpressionMutationnonePromoterSFFVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
ESR1-luciferase
Plasmid#113351Purposeevaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 geneDepositorInsertESR1-enhancer (ESR1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only