Addgene

We narrowed to 35,172 results for: lat

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pGEX6p-1-UFBP1 M1

Plasmid

#204559

Purpose

Expresses GST-tagged UFBP1 M1 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Use

Tags

Expression

Bacterial

Mutation

28-314aa

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pQCXIH FRB-BFP

Plasmid

#202425

Purpose

TagBFP FKBP-rapamycin binding (FRB) dimerization domain

Depositor

David Bryant

Article

Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

Insert

FKBP-rapamycin binding (FRB) dimerization domain

Use

Lentiviral

Tags

TagBFP

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmThor_50-83-P66KP71Q-GB1_AD

Plasmid

#148516

Purpose

Bacterial Expression of DmThor_50-83-P66KP71Q

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmThor_50-83-P66KP71Q (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmThor-del76-84_Z

Plasmid

#148190

Purpose

Bacterial Expression of DmThor-del76-84

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-del76-84 (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NvM-DmThor-del76-84-Y54AM59A_Z

Plasmid

#148191

Purpose

Bacterial Expression of DmThor-del76-84-Y54AM59A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-del76-84-Y54AM59A (Thor Fly)

Use

Tags

Expression

Bacterial

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-CLL78AAA-V5His6_Z

Plasmid

#148165

Purpose

Insect Expression of DmThor-CLL78AAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-CLL78AAA (Thor Fly)

Use

Tags

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-RGTtoAAA-V5His6_Z

Plasmid

#148166

Purpose

Insect Expression of DmThor-RGTtoAAA

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-RGTtoAAA (Thor Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-Y54AM59A-V5His6_Z

Plasmid

#148167

Purpose

Insect Expression of DmThor-Y54AM59A

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-Y54AM59A (Thor Fly)

Use

Tags

Expression

Insect

Mutation

two silent mutations compared to the sequence giv…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-del76-84-V5His6_Z

Plasmid

#148152

Purpose

Insect Expression of DmThor-del76-84

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-del76-84 (Thor Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmThor-del76-85-V5His6_T

Plasmid

#147613

Purpose

Insect Expression of DmThor-del76-85

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

DmThor-del76-85 (Thor Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HseIF4G_571-676-GB1_AG

Plasmid

#148745

Purpose

Bacterial Expression of HseIF4G_571-676

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G_571-676 (EIF4G1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HseIF4G_592-653_AG

Plasmid

#148746

Purpose

Bacterial Expression of HseIF4G_592-653

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G_592-653 (EIF4G1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-β-catenin_S23A,T40A,T41A,T112A

Plasmid

#190824

Purpose

For mammalian expression of β-catenin with 4 O-GlcNAc sites mutated (S23A, T40A, T41A and T112A) and GFP-tag on its N-terminus.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

β-catenin (CTNNB1 Chicken)

Use

Tags

GFP

Expression

Mammalian

Mutation

S23A, T40A, T41A, T112A

Promoter

CMV

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676-GB1_AD

Plasmid

#148512

Purpose

Bacterial Expression of DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676 (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Flnc

Plasmid

#174870

Purpose

CRISPR vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Flnc (Flnc Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Coexpressed Free PcPTS

Plasmid

#182483

Purpose

Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Inserts

FPPS
PTS

Use

Synthetic Biology; Metabolic engineering

Tags

Expression

Yeast

Mutation

Promoter

GAL10 and GAL7

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Linked Free NES (AG4TGGA)2

Plasmid

#182488

Purpose

Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Insert

FPPS-NES

Use

Synthetic Biology; Metabolic engineering

Tags

Expression

Yeast

Mutation

Promoter

GAL10

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Linked Free (PT)4P

Plasmid

#182490

Purpose

Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Insert

FPPS-NES

Use

Synthetic Biology; Metabolic engineering

Tags

Expression

Yeast

Mutation

Promoter

GAL10

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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ABHD2-3'UTR-Del-1

Plasmid

#190095

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR delete-1 (ABHD2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

deleted the base pairs 6704-6709 refer to NM_0070…

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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ABHD2-3'UTR-Del-2

Plasmid

#190096

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR delete-2 (ABHD2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

deleted the base pairs 7122-7129 refer to NM_0070…

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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