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Showing: 3841 - 3860 of 5021 results

  3. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

    Plasmid
    #198569
    Purpose
    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector
    Depositor
    Monther Abu-Remaileh
    Article
    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi
    Insert
    SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
    Use
    Retroviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    changed glutamic acid E164 to lysine (E164K)
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    198569-plasmid-map-sequence-id-438870

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  5. pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH

    Plasmid
    #182425
    Purpose
    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteins
    Depositor
    Dan Mulvihill
    Article
    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396
    Inserts
    VNp-LZ-mNeongreen-hGH (GH1 Human)
    VNp-LZ
    Use
    Tags
    Vesicle Nucleating peptide (VNp) and Vesicle Nucl…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    182425-plasmid-map-sequence-id-360468

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  9. REV human OCT4 TALEN

    Plasmid
    #52413
    Purpose
    TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)
    Depositor
    Jacob Hanna
    Article
    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi
    Insert
    HD HD HD HD HD NI NG NG HD HD NG NI NN NI NI NN NN
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    CAGGS
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

    Plasmid
    #196185
    Purpose
    Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Use
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Mutation
    changed Serine 9 to Alanine
    Promoter
    Tac
    Available since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196185-plasmid-map-sequence-id-383261

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  17. pdCas9-M-G6

    Plasmid
    #73437
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor G6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73437-plasmid-map-sequence-id-135336

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  18. pdCas9-M-3A2

    Plasmid
    #73435
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3A2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73435-plasmid-map-sequence-id-135340

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  20. mVenus-proα2(I)-G610C

    Plasmid
    #119839
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mVenus between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Article
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119839-plasmid-map-sequence-id-233845

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Showing: 3841 - 3860 of 5021 results