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Showing: 3821 - 3840 of 13821 results

  1. pET151-Vps4(101-437)-Hcp1 K321A/R325A

    Plasmid
    #89287
    Purpose
    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1
    Depositor
    Chris Hill
    Article
    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1
    Insert
    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    Use
    Tags
    6xHis-V5-TEV and Hcp1
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-100, mutated Lys-321 and Ar…
    Promoter
    T7
    Available since
    April 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    89287-plasmid-map-sequence-id-175359

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  5. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

    Plasmid
    #169135
    Purpose
    E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.
    Depositor
    Michael Nash
    Article
    Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi
    Insert
    Coh7
    Use
    Tags
    3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    169135-plasmid-map-sequence-id-361163

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  6. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

    Plasmid
    #218973
    Purpose
    Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 June 24. doi: 10.1016/j.m
    Insert
    TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutant SRSF1 binding motif (AGTCTCCT)
    Promoter
    RSV LTR
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218973-plasmid-map-sequence-id-436111

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  7. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

    Plasmid
    #208626
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 191~307, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208626-plasmid-map-sequence-id-415912

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  8. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid
    #208627
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 299~398, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208627-plasmid-map-sequence-id-415915

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  9. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid
    #208629
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    Use
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 212~285, other residues not included
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208629-plasmid-map-sequence-id-415924

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  12. pET-15b(+) Mortierella verticillate (delta prenylation motif)

    Plasmid
    #170788
    Purpose
    Expresses pET-15b(+) Mortierella verticillate (delta prenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Mortierella verticillate PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    170788-plasmid-map-sequence-id-337769

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  13. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid
    #170789
    Purpose
    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Toxoplasma gondii PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    170789-plasmid-map-sequence-id-337771

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  14. pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

    Plasmid
    #166422
    Purpose
    Express pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Plasmodium falciparum PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lac1
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    166422-plasmid-map-sequence-id-329461

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  18. pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)

    Plasmid
    #166415
    Purpose
    Express pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)
    Depositor
    Kalle Gehring
    Article
    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi
    Insert
    Acytostelium subglobosum PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lac1
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    166415-plasmid-map-sequence-id-329462

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Showing: 3821 - 3840 of 13821 results