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Plasmid#103031PurposeLentiviral expression of mRuby2-tagged MRLC with puromycin selectionDepositorInsertMRLC1 (MYL9 Human)
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-SARS-CoV-2 5'UTR-Luciferase
Plasmid#191480PurposeLuciferase reporter for SARS-CoV-2 5' UTRDepositorInsertSARS-CoV-2 5' UTR
UseLentiviral and LuciferaseTagsExpressionMutationcontains SARS-CoV-2 5' UTRPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
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AOI-WT-Cas9-sg-mouse Suz12-F1-GFP
Plasmid#91881PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12DepositorInsertgRNA targeting Suz12 (Suz12 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPv2_FDX1-2
Plasmid#184489PurposeLentivirus all in one CRISPR vector targeting human FDX1 geneDepositorInsertFDX1 (FDX1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HA-LMW-FGF2
Plasmid#157663PurposeExpresses FGF2 in mammalian cellDepositorInsertFibroblast growth factor 2 (FGF2 Human)
UseTagsHA tagExpressionMammalianMutationdeleted amino acid 1-134PromoterAvailable sinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 A82V 2014 EBOV Delta-Mucin-Like-Domain
Plasmid#86022PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationLacks mucin-like domain; Changed alanine 82 to va…PromoterCMV IEAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
p23-NES-PguCas13b-msfGFP-NES-Flag
Plasmid#165072Purposeoverexpression of PguCas13b in human cellsDepositorInsertPguCas13b
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23 A82V 2014 EBOV + Mucin-Like Domain
Plasmid#86016PurposeEbola Virus (Makona) Glycoprotein in CMV driven mammalian expression vectorDepositorInsertMakona Ebola Virus Glycoprotein
UseTagsExpressionMammalianMutationChanged alanine 82 to valinePromoterCMV IEAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD4 (OTU, aa 22-196)
Plasmid#61413PurposeExpresses human OTUD4 (OTU domain) in E. coli.DepositorInsertOTUD4 (OTU domain-containing protein 4) (OTUD4 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-21 and aa 197-1114.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR/D614G
Plasmid#164570PurposeSARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)DepositorInsertSpike (S-RRAR-D614G variant)
UseTags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationD614GPromoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-mScarlet-SARS-CoV-2-SL1 alone
Plasmid#191485PurposeLentiviral expression of SARS-CoV-2 5_ UTR SL1 aloneDepositorInsertSARS-CoV-2 5' UTR SL1 alone (NEWENTRY Severe acute respiratory syndrome coronavirus 2)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human HoxB4-IRES-YFP
Plasmid#91889PurposeRetroviral expression of human HoxB4DepositorInsertHoxB4 (HOXB4 Human)
UseRetroviralTagsIRES-EYFPExpressionMammalianMutationPromoterMMLV gagAvailable sinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-G6
Plasmid#73437PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.DepositorInsertRepressor G6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-mouse Gfi1-GFP
Plasmid#91892Purpose3rd generation lentiviral expression of mouse Gfi1DepositorInsertGfi1 (Gfi1 Mouse)
UseLentiviralTagsIRES-EGFPExpressionMammalianMutationPromoterUnknownAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-3A2
Plasmid#73435PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.DepositorInsertRepressor 3A2 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterConstitutive wild-type S. pyogenes promoterAvailable sinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
FGFR3_HUMAN_D0
Plasmid#79731PurposeThis plasmid encodes the kinase domain of FGFR3. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFGFR3 (FGFR3 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1D_HUMAN_D0
Plasmid#79723PurposeThis plasmid encodes the kinase domain of KC1D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertKC1D (CSNK1D Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPv2_FDX1-3
Plasmid#184490PurposeLentivirus all in one CRISPR vector targeting human FDX1 geneDepositorInsertFDX1 (FDX1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPv2_FDX1-1
Plasmid#184488PurposeLentivirus all in one CRISPR vector targeting human FDX1 geneDepositorInsertFDX1 (FDX1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only